不同病原体感染致肺炎患者血清ADA、PAB、LTB4水平变化情况及与患者肺泡上皮损伤程度的关系

Changes of serum ADA,PAB and LTB4 levels in patients with pneumonia caused by different pathogens and their relationship with severity of alveolar epithelial injury

目的探讨不同病原体感染致肺炎患者血清腺苷酸脱氨酶(ADA)、前清蛋白(PAB)、白三烯B4(LTB4)水平变化情况及其与患者肺泡上皮损伤程度的关系。方法选取2017年6月至2019年12月该院收治的肺炎患者125例作为研究对象,其中病毒感染39例(病毒感染组),细菌感染37例(细菌感染组),支原体感染49例(支原体感染组),另选取125例门诊健康体检者作为对照组,比较4组患者血清ADA、PAB、LTB4、肺表面活性物质相关蛋白A(SP-A)表达水平,采用Western blot检测SP-A表达水平,分析不同肺泡上皮损伤程度患者血清ADA、PAB、LTB4水平的差异,并对上皮损伤程度与血清ADA、PAB、LTB4水平进行相关性分析。结果4组患者血清ADA、PAB、LTB4、SP-A水平比较,差异均有统计学意义(P<0.05);血清ADA、LTB4、SP-A水平由高至低依次为细菌感染组、支原体感染、病毒感染组,PAB水平由高至低依次为病毒感染组、支原体感染、细菌感染组。结论细菌感染患者肺泡上皮损伤程度较高,检测血清ADA、PAB、LTB4水平有助于了解患者肺泡上皮损伤情况。

Objective To investigate the changes of serum ADA,PAB and LTB4 levels in the patients with pneumonia caused by different pathogens and their relationship with the degree of alveolar epithelial injury.Methods One hundred and twenty-five patients with pneumonia treated in this hospital from June 2017 to December 2019 were selected as the research subjects,including 39 cases of viral infection(viral infection group),37 cases of bacterial infection(bacterial infection group)and 49 cases of mycoplasma infection(mycoplasma infection group).Other 125 subjects undergoing healthy physical examinations in the outpatients department were selected as the control group.Serum ADA,PAB,LTB4 and SP-A expression levels were compared among the four groups.The Western blot was used to detect the expression level of SP-A.The differences in the levels of serum ADA,PAB and LTB4 of the patients with different degrees of alveolar epithelial injury were analyzed,and the correlation between the epithelial injury degree with serum ADA,PAB and LTB4 levels was analyzed.Results There were statistically significant differences in serum ADA,PAB,LTB4 and SP-A levels among the four groups(P<0.05).The levels of serum ADA,LTB4 and SP-A from high to low were in turn the bacterial infection group,mycoplasma infection group and viral infection group,and the levels of PAB levels from high to low were in turn was the viral infection group,mycoplasma infection group and bacterial infection group.Conclusion The alveolar epithelium in the patients with bacterial infection has a higher degree of damage.Detecting serum ADA,PAB and LTB4 levels ie helpful to understand the damage situation of alveolar epithelium.