海藻酸钠/羧甲基微胶囊对副干酪乳杆菌生存率影响的研究

Effect of sodium alginate/carboxymethyl microcapsule on the survival rate of Lactobacillus paracasei

为探究提高食品中副干酪乳杆菌在胃肠道存活的方法,本试验以海藻酸钠(Alg)和N,O-羧甲基壳聚糖(CMCS)为囊材,采用静电液滴法和传统挤出法分别制备海藻酸钠-羧甲基壳聚糖微胶囊;以Alg和CMCS的不同配比为影响因素,测定其包埋产率、抗酶活性、过胃肠道模拟液后的存活率以及耐储存性。对微胶囊进行傅利叶红外分析和扫描电镜分析。结果表明:静电液滴法能够明显改善微胶囊粒径大小;Alg和CMCS比例为1:1时(E-AC)微胶囊包埋产率最大,为(92.20±1.02)%。与游离的副干酪乳杆菌相比,过胃肠道模拟液后(SIF)后,E-AC微胶囊包埋的益生菌存活率显著提高(P<0.01),存活量为(7.6±0.65)Log_(10) cfu/mL。综上,采用静电液滴法制备的微胶囊与传统挤出法相比粒径较小,存活率无统计学差异。储存性试验表明,E-AC在储存4周后,益生菌存活率较高。综上,Alg和CMCS为壁材制备的微胶囊在一定程度上改善了益生菌的存活率和耐储存性。

In order to improve the survival of Lactobacillus paracasei in the gastrointestinal tract of animal feed,sodium alginate(Alg)and N,O-carboxymethyl chitosan(CMCS)were used as capsule materials to prepare sodium alginate-carboxymethyl chitosan microcapsules by electrostatic liquid dropping method and traditional dripping method,respectively.The different proportions of Alg and CMCS were used as the influencing factors to determine the embedding yield,anti-enzyme activity,survival rate and storage tolerance of the simulated solution.The microcapsules were analyzed by Fourier transform infrared(FTIR)and scanning electron microscopy(SEM).The results showed that the particle size of microcapsules could be significantly improved by electrostatic droplet method.When the ratio of Alg to CMCS was 1:1,the embedding yield of E-AC was the highest,which was(92.20±1.02)%.Compared with free Lactobacillus paracasei,the survival rate of probiotics encapsulated in E-AC microcapsules was significantly increased(P<0.01)after passing through gastrointestinal simulated solution(SIF),and the survival rate was(7.6±0.65)Log_(10) cfu/mL.In conclusion,the particle size of microcapsules prepared by electrostatic droplet method was smaller than that by traditional droplet method,and there was no statistical difference in survival rate(P>0.05).Storage experiments showed that E-AC had a higher probiotics survival rate after 4 weeks of storage.In conclusion,the microcapsules prepared by Alg and CMCS as wall materials could improve the survival rate and storage endurance of probiotics to a certain extent.