miR-3679-5p通过抑制FOXM1表达对食管癌KYSE30细胞增殖和凋亡的影响

Effect of miR-3679-5p on the proliferation and apoptosis of esophageal cancer KYSE30 cells by inhibiting the expression of FOXM1

目的探讨微小RNA(miRNA)-3679-5p通过调控叉头框蛋白M1(forkhead box protein M1,FOXM1)的表达对食管癌KYSE30细胞增殖和凋亡的影响。方法根据对KYSE30细胞的处理,实验分为NC组(转染阴性对照模拟物)和miR-3679-5p组(转染miR-3679-5p模拟物)。实时荧光定量聚合酶链反应(RT-qPCR)检测各组KYSE30细胞中miR-3679-5p表达变化。克隆形成实验和双染流式细胞术分别检测KYSE30细胞增殖和凋亡变化。生物信息学方法预测miR-3679-5p的靶基因。RT-qPCR和Western blotting检测靶基因表达变化。结果与NC组相比,miR-3679-5p组KYSE30细胞中miR-3679-5p表达显著上调[(8.65±0.68)比(1.09±0.31)](P<0.01),KYSE30细胞增殖活力显著降低[(48.46±12.79)个比(105.70±13.57)个](P<0.01),细胞凋亡率显著增加[(19.37±2.66)%比(5.48±1.34)%](P<0.01)。FOXM1可能是miR-3679-5p的靶基因。与NC组相比,miR-3679-5p组KYSE30细胞中FOXM1基因表达显著降低(P<0.01)。结论miR-3679-5p可能通过靶向下调FOXM1表达,抑制食管癌KYSE30细胞增殖,促进KYSE30细胞的凋亡。

Objective To explore the effect of microRNA(miR)-3679-5p on the proliferation and apoptosis of esophageal cancer KYSE30 cells by regulating the expression of forkhead box protein M1(FOXM1).Methods According to the treatment methods of KYSE30 cells,the cells was divided into a NC group(transfected with negative control mimic)and a miR-3679-5p group(transfected with miR-3679-5p mimic).Real-time fluorescent quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression changes of miR-3679-5p in KYSE30 cells in each group.Clone formation experiment and double staining flow cytometry were used to detect the changes of proliferation and apoptosis of KYSE30 cells.Bioinformatic method was used to predict the target gene of miR-3679-5p.RT-qPCR and Western blotting were used to detect the expression changes of the target gene.Results Compared with those in the NC group,the expression of miR-3679-5p in KYSE30 cells in the miR-3679-5p group was significantly up-regulated[(8.65±0.68)vs.(1.09±0.31)](P<0.01),the proliferation activity of KYSE30 cells was significantly reduced[(48.46±12.79)vs.(105.70±13.57)](P<0.01),and the apoptosis rate was significantly increased[(19.37±2.66)%vs.(5.48±1.34)%](P<0.01).FOXM1 might be the target gene of miR-3679-5p.Compared with that in the NC group,the expression of FOXM1 gene in KYSE30 cells in the miR-3679-5p group was significantly reduced(P<0.01).Conclusion miR-3679-5p may inhibit the proliferation of esophageal cancer KYSE30 cells and promote the apoptosis of KYSE30 cells by inhibiting the expression of FOXM1.