基于网络药理学探讨麻杏二三汤治疗慢性阻塞性肺疾病作用机制

Mechanism research of Maxing-Ersan Decoction in the treatment of COPD based on network pharmacology

目的利用网络药理学探讨麻杏二三汤治疗COPD的作用靶点、信号通路,揭示麻杏二三汤治疗COPD多靶点、多通路的干预作用机制。方法借助TCMSP筛选麻杏二三汤的有效成分及相应靶点,利用GeneCards数据库筛选COPD疾病相关靶点。运用STRING数据库进行蛋白相互作用(protein-protein interaction,PPI)网络分析。通过Cytoscape构建化合物靶点网络、PPI网络,利用Draw Venn Diagram网站绘制韦恩图筛选交集靶点,运用R软件对麻杏二三汤作用靶点进行GO功能富集分析及KEGG通路分析。结果共得出麻杏二三汤101个活性成分,对应250个靶点,包括与COPD相关靶点214个。通过GO及KEGG富集分析,筛选出与麻杏二三汤主要成分相关的信号通路有48条。结论基于网络药理学分析得出麻杏二三汤有效化学成分,发现麻杏二三汤可能通过炎症反应、调节免疫等多靶点、多途径作用于COPD。

Objective To explore the targets and signal pathways of Maxing-Ersan Decoction in the treatment of COPD by using network pharmacology,and to reveal the mechanism of intervention of multiple targets and multiple pathways of Maxing-Ersan Decoction in the treatment of COPD.Methods The active ingredients and corresponding targets of Maxing-Ersan Decoction were screened with the help of TCMSP database,and the targets related to COPD disease were screened with GeneCards database.The STRING database was used for protein-protein interaction(PPI)network analysis.By using Cytoscape to build compound target network and PPI network;by using Draw Venn Diagram website to draw a Venn diagram,and using R software to perform gene ontology(GO)functional enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG).Results A total of 101 active ingredients were obtained,with 250 corresponding targets,including 214 targets related to COPD.Through GO and KEGG enrichment analysis,all together 48 signal pathways related to the main components of Maxing-Ersan Decoction were screened out.Conclusion Based on the network pharmacological analysis,the effective chemical components of Maxing-Ersan Decoction were found,and it was found that Maxing-Ersan Decoction may act on COPD through multiple targets and multiple pathways such as inflammation and immune regulation.