罗氏化学发光法与微粒子化学发光法检测肿瘤血清CA153、CA125、CA199的一致性评价

Evaluation of Consistency of Roche Electrochemiluminescence Method and Chemiluminescence Microparticle Immunoassay in Detecting Tumor Serum CA153,CA125 and CA199

目的对罗氏Cobase602电化学发光法检测乳腺癌和肝癌患者CA125、CA153以及CA199三种肿瘤标志物联合检测进行性能验证,并对电化学发光法和化学发光微粒子法检测CA125、CA153以及CA199等指标在肿瘤诊断中的结果进行一致性评价。方法分别使用以上两种化学发光法检测我院120例送检肿瘤的血清样本的CA199、CA125以及CA153等指标,并且对这两种方法得到的最终检测结果进行一致性评价。结果对于血清中的CA125、CA153以及CA199在低浓度(0.0~35.0 U/mL)情况下两种方法的测定结果差异性显著;CA125、CA153以及CA199在中浓度和高浓度情况下(35.1~500.0 U/mL、>500.0 U/mL)两种检测方法的测定结果差异无统计学意义(P>0.05)。结论罗氏Cobase602电化学发光法和化学发光微粒子法检测CA125、CA153以及CA199指标在高值部分一致性良好,虽然没有普遍性,但可以看出CA125、CA153、CA199检测系统之间数据在低浓度不具有一致性,在中浓度和高浓度下具有一致性。

Objective To verify the performance ofcombined detection of three tumor markers of CA125,CA153 and CA199 by Roche Cobase602 electrochemiluminescence method in breast cancer and liver cancer patients,and to evaluate the consistency of results of electrochemiluminescence method and chemiluminescence microparticle immunoassay for detecting CA125,CA153 and CA199 in tumor diagnosis.Methods The above two chemiluminescence methods were used to detect the CA199,CA125,and CA153 of the120 tumor serum samples submitted for examination in our hospital,and the consistency of the final test results obtained by these two methods was evaluated.Results For the serum levels of CA125,CA153 and CA199 at low concentrations(0.0-35.0 U/ml),there were statistically significant differences in the results of the two methods.There were no significant differences in the detection results of the two detection methods under the condition of CA125,CA153 and CA199 at middle concentration and high concentration(35.1-500.0 U/ml,>500.0 U/ml),P>0.05.Conclusion Roche Cobase602 electrochemiluminescence method and chemiluminescencemicroparticle immunoassay have good consistency in the high-value parts of ndicators such as CA125,CA153 and CA199.Although there is no universality,it can be seen that the data among the CA125,CA153 and CA199 detection systems at low concentration are not consistent,and are consistent at middle and high concentrations.