油橄榄WRKY转录因子的全基因组鉴定与分析

Genome-wide identification and analysis of olive WRKY transcription factor

以干旱和水淹胁迫下的‘佛奥’和‘TYZ-1号’2个品种苗木为试材,基于油橄榄全基因组数据,鉴定出106个油橄榄WRKYGQK七肽结构蛋白(WRKY)基因家族成员.利用生物信息学软件进行结构和功能预测,结合油橄榄水分(干旱、水淹)胁迫基因表达谱,分析出7个WRKY转录因子(WRKY14、WRKY51、WRKY77、WRKY91、WRKY66、WRKY1、WRKY92)在‘佛奥’和‘TYZ-1号’中均有高度表达且共同参与干旱和水淹胁迫调控,推测这7个基因可能是油橄榄水分胁迫的主要调节因子.将WRKY蛋白与已知干旱调控的WRKY转录因子构建系统进化树,结果显示,7个与胁迫相关的转录因子中的4个(WRKY14、WRKY51、WRKY91、WRKY92)与已知干旱调控的WRKY基因相似度更高,推测这4个WRKY转录因子可能是参与‘TYZ-1号’干旱调控的主要基因.

106 members of the gene family encoding WRKYGQK heptapeptide structural protein(WRKY) transcription factors were identified based on the genomic data of 2 cultivars of Olea europaea L., ‘Frantoio’ and ‘TYZ-1’, under drought and waterlogging stress. Based on the structure and function of olive WRKY transcription factors predicted using bioinformatics software and the gene expression profile under drought and waterlogging conditions, 7 WRKY transcription factors(WRKY14, WRKY51, WRKY77, WRKY91, WRKY66, WRKY1, and WRKY92) which were highly expressed in ‘Frantoio’ and ‘TYZ-1’ were considered to be the main regulatory factors for olive under water stress. According to the phylogenetic tree of WRKY proteins from olive and Arabidopsis, 4 WRKY(WRKY14, WRKY51, WRKY91, and WRKY92) showed high levels of similarities with Arabidopsis WRKY transcription factors, which were known to be involved under drought stress. Therefore, it can be speculated that the 4 WRKY transcription factors were the main genes involved in drought regulation of ‘TYZ-1’.