转录组学分析低温胁迫对西方蜜蜂工蜂6日龄幼虫基因表达的影响

Transcriptomic analysis of gene expression in 6-day-old Apis mellifera worker larvae under low temperature stress

温度是影响蜜蜂发育的关键因子之一.为研究低温胁迫对西方蜜蜂幼虫基因表达的影响,利用高通量转录组测序技术分别对最适发育温度和低温处理的西方蜜蜂工蜂6日龄幼虫的基因表达情况进行分析,获得差异表达基因(DEGs),并对DEGs进行GO功能分类和KEGG富集分析.结果显示,低温处理后共检测到98个DEGs.GO功能分类显示,DEGs富集在21条二级GO功能注释上,富集最多DEGs的GO功能注释为结合、细胞进程、代谢进程、催化活性;KEGG富集分析显示,上调和下调DEGs分别富集在25和3条通路上,主要包括甘氨酸-丝氨酸-苏氨酸代谢、核黄素代谢、昆虫激素生物合成等代谢通路,以及与信号转导相关的Hippo、Notch等信号通路.qPCR结果显示,转录组测序获得的DEGs IP3K、Ex、CYP18A1、GOD、GCDH表达差异显著.本研究结果揭示西方蜜蜂工蜂6日龄幼虫主要通过提高抗氧化能力和减慢发育速度应对低温.

Temperature is one of the most important factors affecting the development of honeybee. To study the effect of low temperature stress on Apis mellifera larvae, the differentially expressed genes(DEGs) in 6-day-old A.mellifera larvae grew in the optimal temperature and low temperature state were studied by high throughput transcriptomic sequencing. The putative molecular mechanism in response to low temperature stress was analyzed by gene ontology(GO) functional classification and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis. A total of 98 DEGs were detected after low temperature stress. They were enriched in 21 GO categories, which participated in various binding, cellular, metabolic processes and catalytic activity. KEGG analysis further showed that the up-regulated and down-regulated DEGs were enriched in 25 and 3 pathways, respectively, which regulated the metabolisms of glycine-serine-threonine and riboflavin, hormone biosynthesis, and Hippo and Notch signaling pathways. Results of qPCR verified that the expressions of DEGs like IP3K, Ex, CYP18A1, GOD and GCDH were significantly different under low temperature stress. The study indicated that the 6-day-old A.mellifera larvae responded to low temperature stress by enhanced antioxidative ability and decelerated developmental rate.