外源性激素茉莉酸甲酯对鹅掌草中三萜皂苷flaccidosideⅡ含量积累作用的分析研究

Effects of methyl jasmonate on the accumulation of triterpenoid saponin flaccidosideⅡin Anemone flaccida

目的观察施用外源激素茉莉酸甲酯对鹅掌草中三萜皂苷flaccidosideⅡ含量积累的影响。方法采用不同浓度的茉莉酸甲酯激素刺激鹅掌草根茎,分别为茉莉酸甲酯高浓度5mmol/L组、茉莉酸甲酯中浓度0.5mmol/L组、茉莉酸甲酯低浓度0.05mmol/L组、正常组共4组连续4周对鹅掌草进行灌根处理经液氮研磨提取后用液相质谱联用技术进行含量分析,研究外源激素茉莉酸甲酯对鹅掌草三萜皂苷flaccidosideⅡ的含量影响。结果采用外源激素茉莉酸甲酯刺激鹅掌草根茎使其flaccidosideⅡ含量增加flaccidosideⅡ在3组平行实验中含量具有明显差异,正常组含量0.0529%、低剂量组含量0.2809%、中剂量组含量0.3503%、高剂量组含量0.3264%。结论茉莉酸甲酯灌根处理鹅掌草根茎在一定程度上对鹅掌草中三萜皂苷flaccidosideⅡ含量积累具有促进作用。

Objective To observe the effect of exogenous hormone methyl jasmonate(MeJA)on the accumulation of triterpenoid saponin flaccidosideⅡin Anemone flaccida.Methods Different concentrations of MeJA hormone were used to stimulate the roots of Anemone flaccida,which were divided into three groups:high concentration of MeJA group(0.5 mmol/L),low concentration of MeJA group(0.05 mmol/L)and normal group.The roots of Anemone flaccida were irrigated for 4 weeks.After grinding and extracting with liquid nitrogen,liquid phase mass spectrometry chromatography-mass spectrometry(LC-MS)was used to detect the contents of triterpenoid saponin flaccidosideⅡin each group.Results The content of flaccidosideⅡincreased by stimulating the roots of Anemone flaccida with exogenous hormone MeJA,the content of flaccidosideⅡin each group was significantly different,the content of flaccidosideⅡwas 0.0529%in normal group,0.2809%in low dose group,0.3503%in middle dose group,and 0.3264%in high dose group.Conclusion The root-irrigating treatment with MeJA promotes the accumulation of triterpenoid saponin flaccidosideⅡin Anemone flaccida to a certain extent.