鳕鱼鳔胶原肽对H_(2)O_(2)诱导2BS细胞早期衰老的保护作用

Protective Effect of SWP on Premature Senescence 2BS Cells Induced by H_(2)O_(2)

目的:以人胚肺二倍体成纤维细胞(2BS)为研究对象,探讨鳕鱼鳔胶原肽对H_(2)O_(2)诱导2BS细胞早期衰老的保护作用。方法:采用H_(2)O_(2)诱导2BS细胞建立早期衰老细胞模型,给药组加入不同浓度的鳕鱼鳔胶原肽,以白藜芦醇作为阳性对照,对细胞的增殖率、ROS活性氧水平、细胞凋亡情况、β-半乳糖苷酶含量及过氧化物酶含量进行检测。结果:与模型组细胞相比,经白藜芦醇与鳕鱼鳔胶原肽处理后的细胞存活率提高,ROS活性氧水平、细胞凋亡率与β-半乳糖苷酶含量降低,SOD与CAT活力提高,MDA含量降低,且鳕鱼鳔胶原肽保护效果与给药剂量呈正相关。结论:鳕鱼鳔胶原肽能够有效缓解H_(2)O_(2)诱导所造成的早期衰老,在一定程度上降低氧化损伤。

Objective: The protective effects of swimming bladder peptides(SWP) from cod on premature senescence human embryonic lung diploid fibroblast(2BS) cells were studied. Methods: 2BS cells were used to establish the model of precocious senescence induced by H_(2)O_(2). Different concentrations of SWP were added to the precocious senescence cells and resveratrol was used as positive control. The proliferation rate, the cellular apoptosis, the content of β-galactosidase,the level of ROS and the content of peroxidase were measured. Results: Compared with model control group, the survival rates of cells treated with SWP and resveratrol was improved, the ROS level and cellular apoptosis were decreased. Besides, the activities of SOD and CAT were increased and the content of MDA was decreased. The protective effect of swimming bladder peptides was positively correlated with the dose administrated. Conclusion: SWP possessed the ability of relieving premature senescence of 2BS cells induced by H_(2)O_(2) and partly reducing oxidative damage.