单细胞捕捉微流控芯片设计及流场分析

Design and Flow Field Analysis of Single-Cell Capture Microfluidic Chip

聚合物单细胞捕获微流控芯片中流场分布对细胞捕获有着重要意义,是芯片结构设计的一个关键因素。在聚合物单细胞捕获微流控芯片中,不同尺寸微坝结构对细胞捕获效率有影响。针对成骨细胞的捕获与培养设计了3种尺寸的微坝结构,并对成骨细胞捕获和培养过程芯片内部流场进行了模拟分析,模拟结果显示下端开口尺寸为10μm的微坝结构内部流速最小,故其流体对细胞产生的作用力越小,细胞变形程度越小,细胞越容易被留在微坝中,从而被捕获。为之后的实验提供了一定的指导作用。

The flow field distribution in polymer single-cell capture microfluidic chips is of great significance for cell capture and is a key factor in chip structure design.In the polymer single-cell capture microfluidic chip,the different size of the microdam structure has an effect on the efficiency of cell capture.Based on osteoblast capture and develop,three sizes of dam structure were designed,and the flow field in the chip during osteoblast capture and culture was simulated and analyzed,simulation results show that the lower end of the opening size of 10 microns minimum dam structure internal flow velocity,so the fluid to the cells to produce force is smaller,the smaller the cell deformation degree,cells more easily in the dam,which is captured.It provides some guidance for the later experiment.