基于二次回归旋转正交法优化香菇多糖的提取工艺

Optimization of Extraction Process of Polysaccharides from Lentinus edodes Based on Quadratic Regression Rotation Orthogonal Method

为优化香菇多糖的提取工艺,以香菇多糖的提取率为指标,基于单因素法和二次回归旋转正交法分别对浸提时间、料液比、超声温度、超声时间、超声功率对香菇多糖提取的影响进行分析。结果表明,干香菇中多糖的最佳提取工艺方案:料液比为1∶40,浸提时间为0.5 h,超声温度为60℃,超声时间为20 min。验证试验证明,香菇多糖的提取率为15.86%,与试验预估值15.97%基本吻合。因此,此工艺可稳定提高香菇多糖的提取率。

In order to optimize the extraction process of polysaccharides from Lentinus edodes,the effects of extraction time,solid-liquid ratio,ultrasonic temperature,ultrasonic time and ultrasonic power on the extraction rate of polysaccharides from Lentinus edodes are analyzed by single factor method and quadratic regression rotation orthogonal method.The results show that the optimal extraction process is as follows:the solid-liquid ratio is 1∶40,the extraction time is 0.5 h,the ultrasonic temperature is 60℃,the ultrasonic time is 20 min.The validation test results show that the extraction rate of polysaccharides from Lentinus edodes is 15.86%,which is basically consistent with the estimated value of 15.97%.Therefore,this process can stably improve the extraction rate of polysaccharides from Lentinus edodes.