生防菌株HG18基因组信息分析与抗菌功能基因挖掘

Genomic Information Analysis and Antibiotic Functional Gene Excavation of Biocontrol Strain HG18

贝莱斯芽胞杆菌(Bacillus velezensis)HG18是一株低温生防菌株,能够分泌抗菌物质。为挖掘和利用其抗菌功能基因,服务农业生产,采用二、三代相结合测序技术,对其进行全基因组测序,获得菌株完整基因组序列。基因组全长4461844 bp,包含一个染色体和一个质粒,GC含量44.06%,编码4643个基因,编码基因总长度3893994 bp,占基因组87.27%。发现6个几丁质降解相关基因,2个葡聚糖酶基因和1个壳聚糖酶基因,2个脂肽类抗菌物质芬芥素与表面活性素合成基因簇,2个细菌素subtilin和bacillolysin合成基因。研究为提高抗菌物质产量的菌株定向遗传改造以及植物抗病育种提供基因资源。

Bacillus velezensis HG18,is a cryogenic biocontrol strain,secretes antibiotic substances.In order to excavate and utilize its antibiotic genes and serve agricultural production,the whole genome of the strain was sequenced by the second and third generation sequencing technology,and the complete genome sequence was obtained.The genome size is 4461844 bp,including a chromosome and a plasmid,whose GC content is 44.06%,encoding 4643 genes.The total length of the coding genes is 3893994 bp,accounting for 87.27%of the genome.Six genes related to chitin degradation,two glucanase genes and one chitosanase gene,two gene clusters related to synthesis of fengycin and surfactin,and two genes related to synthesis of subtilin and bacillolysin were found.This study provides gene resources for directional genetic modification of the strain to improve the yield of antibiotic substances and plant disease resistance breeding.