D-二聚体、血管内皮生长因子、泛素连接酶联合检测在C6脑胶质瘤模型大鼠脊髓播散中的应用

The Evaluation Value of D-dimer,VEGF and FBW7 Combined Detection for Spinal Cord Transmission in C6 Glioma Model Rats

目的:了解D-二聚体(D-dimer)、血管内皮生长因子(VEGF)、泛素连接酶(FBW7)联合检测对大鼠胶质瘤疾病诊断、脊髓播散评估的价值。方法:在84只健康Wistar雄性大鼠中随机抽取42只大鼠不做任何处理,纳为对照组;其余42只大鼠成功造模(C6脑胶质瘤模型),纳入胶质瘤大鼠组,并根据是否发生脊髓播撒分为发生脊髓播散组、未发生脊髓播散组。比较正常大鼠和胶质瘤大鼠之间、发生脊髓播散胶质瘤大鼠和未发生脊髓播散胶质瘤大鼠之间D-dimer、VEGF、FBW7含量,分析3项指标串联或并联诊断胶质瘤大鼠脊髓播散的价值,回归分析3项指标用于胶质瘤大鼠脊髓播散评估的价值。结果:胶质瘤大鼠D-dimer、VEGF含量显著高于正常大鼠,而FBW7显著低于正常大鼠(P<0.05)。脊髓播散胶质瘤大鼠D-dimer[(6.72±1.57)pg·mL^(-1)]、VEGF[(191.20±10.64)pg·mL^(-1)]高于未发生脊髓播散胶质瘤大鼠[(5.35±1.49)pg·mL^(-1)、(142.15±9.78)pg·mL^(-1)],脊髓播散胶质瘤大鼠FBW7[(0.49±0.05)pg·mL^(-1)]低于未发生脊髓播散胶质瘤大鼠[(0.58±0.06)pg·mL^(-1)],差异均有统计学意义(P<0.05)。3项指标并联使用诊断灵敏度为96.42%,串联使用时可以获得最高特异度92.86%。3项指标串联、并联使用可以获得最佳诊断效果,约登指数均为0.39。3项指标含量对胶质瘤大鼠脊髓播散影响依次为:VEGF(OR=3.706)、FBW7(OR=2.586)、D-dimer(OR=2.435)。结论:VEGF、FBW7、D-dimer 3项指标可用于大鼠胶质瘤疾病诊断和脊髓播散的评估,串联使用效果较好。

Objective:To understand the value of combined detection of D-dimer,VEGF and FBW7 in the diagnosis and evaluation of spinal cord dissemination of glioma in rats.Methods:84 healthy Wistar male rats were divided into normal rat group(untreated)and glioma rat group(model C6 brain glioma).The differences of D-dimer,VEGF,FBW7 content between normal rats and glioma rats,between rats with spinal glioma and rats without spinal glioma were compared.Three indicators in series or parallel for glioma were analyzed in the rat spinal cord spread the effect of diagnosis.Regression analysis was used to analyze three indicators for assessment of glioma in the rat spinal cord spread effect.Results:The content of D-dimer and VEGF in glioma rats was significantly higher than that in normal rats,while FBW7 was significantly lower than that in normal rats(P<0.05).D-dimer,VEGF of spinal cord disseminated glioma rats were higher than those without spinal cord disseminated glioma.While FBW7 of spinal cord disseminated glioma rats was lower than those without spinal cord disseminated glioma,the differences were statistically significant(P<0.05).The highest sensitivity(96.42%)can be obtained when the three indexes are used in parallel,the highest specificity(92.86%)can be obtained when are used in series.Three indicators can be used in series and in parallel to obtain the best diagnostic effect,and the Youden index is both 0.39.The influence of the content of three indexes on the spinal cord transmission of glioma rats was as follows:VEGF(OR=3.706),FBW7(OR=2.586),D-dimer(OR=2.435).Conclusion:VEGF,FBW7 and D-dimer can be used for the diagnosis of glioma disease and the evaluation of spinal cord spread in rats.The combined use of the three indexes has a good effect.