巴西人参根化学成分的研究

Chemical constituents from the roots of Pfaffia glomerata

目的研究巴西人参Pfaffia glomerata(Spreng)Pedersen根的化学成分。方法巴西人参根95%乙醇提取物采用硅胶、MCI、HPLC制备柱、薄层色谱、ODS进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到23个化合物,分别鉴定为20-羟基脱皮激素(1)、蕨甾酮(2)、豆甾醇(3)、β-谷甾醇(4)、7α-hydroxysitosterol(5)、7β-hydroxysitosterol(6)、胡萝卜苷(7)、齐墩果酸(8)、eupatoric acid(9)、齐墩果酸-28-O-β-D-吡喃葡萄糖酯苷(10)、pfaffine B(11)、3-甲氧基槲皮素(12)、槲皮素(13)、7,4′-dihydroxy-5-methoxydihydroflavone(14)、木樨草素(15)、(+)-丁香脂素(16)、(+)-nyasol(17)、N-反式-阿魏酰酪胺(18)、小檗碱(19)、棕榈酸(20)、10-eicosenoic acid(21)、蔗糖(22)和邻苯二甲酸二丁酯(23)。结论化合物5~6、9、12~23为首次从法菲亚属植物和该植物中分离得到。

AIM To study the chemical constituents from the roots of Pfaffia glomerata(Spreng)Pedersen.METHODS The 95%ethanol extract from P.glomerata was isolated and purified by silica,MCI,preparative HPLC column,thin layer chromatography,and ODS,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Twenty-three compounds were isolated and identified as 20-hydroxyecdysone(1),pterosterone(2),stigmasterol(3),β-sitosterol(4),7α-hydroxysitosterol(5),7β-hydroxysitosterol(6),daucosterol(7),oleanolic acid(8),eupatoric acid(9),oleanolicacid-28-O-β-D-glucopyranoside(10),pfaffine B(11),3-methylquercetin(12),quercetin(13),7,4′-dihydroxy-5-methoxydihydroflavone(14),luteolin(15),(+)-syringaresin(16),(+)-nyasol(17),N-trans-feruloyltyramine(18),berberine(19),palmitic acid(20),10-eicosenoic acid(21),sucrose(22)and dibutyl phthalate(23).CONCLUSION Compounds 5-6,9,and 12-23 are isolated from genus Pfaffia and this plant for the first time.