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CAR-T细胞采集的相关影响因素及细胞免疫功能分析 被引量:1

Analysis of related influencing factors and cellular immune function of CAR-T cell collection
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摘要 目的通过观察血细胞分离机采集CAR-T细胞的临床结果,分析相关影响因素及细胞免疫功能。方法采用COBE Spectra白细胞去除管路对细胞采集组的18例(包括13例自体、5例健康供者)受检者进行产品采集,根据要求采集100 mL产品后进行CAR-T细胞培养,培养成功后进行CAR-T细胞输注。以在我院进行体检的20名健康者为健康对照组。通过分析采集前、后受检者的血常规指标、血糖、血钙、血钾水平及细胞免疫功能(包括Treg细胞及T亚群),并监测采集过程中有无不良反应,来分析CAR-T采集对于机体的影响及对产品质量的把控方法。结果细胞采集组的18例受检者均采集并培养成功,1例输注后在体内扩增失败。采集后,细胞采集组的RBC、HCT、HGB、WBC、MNC百分比、PLT及血钙水平均低于采集前(P<0.01)。采集过程中均无不良反应发生。细胞采集组空腹外周血的CD8^(+)、CD4^(+)CD25^(+)CD127low/CD4^(+)T细胞高于健康对照组,CD4^(+)/CD8^(+)低于健康对照组(P<0.01);采集产品组采集产品的CD8^(+)、CD4^(+)CD25^(+)CD127low/CD4^(+)T细胞以及CD127low/CD127high高于健康对照组,CD4^(+)/CD8^(+)低于健康对照组(P<0.05)。结论为保证CAR-T细胞采集的质量和供者的安全,采集前一定要有良好的血常规指标和血钙水平,并保持良好的免疫平衡状态。 Objective To analyze the related influencing factors and cellular immune function by observing the clinical results of CAR-T cells collected by blood cell separator.Methods The COBE Spectrum leukocyte removal pipeline was used to collect products from 18 subjects(including 13 autologous and 5 healthy donors)in the cell collection group.CAR-T cells were cultured after collecting 100 mL of product according to the requirements.After successful culture,CAR-T cells were infused.The 20 healthy persons who underwent physical examination in our hospital were selected as healthy control group.The influence of CAR-T collection on the body and the control method of product quality were analyzed by analyzing the blood routine indexes,blood glucose,blood calcium,blood potassium levels and cellular immune function(including Treg cells and T subsets)of the subjects before and after collection,and monitoring whether there were adverse reactions during collection.Results All 18 subjects in the cell collection group were collected and cultured successfully,and 1 case failed to expand in vivo after infusion.After collection,the RBC,HCT,HGB,WBC,percentage of MNC,PLT and blood calcium level in the cell collection group were significantly lower than those before collection(P<0.01).No adverse reactions occurred during the collection process.The CD8^(+),CD4^(+)CD25^(+)CD127low/CD4^(+)T cells of fasting peripheral blood in the cell collection group were higher than those in the healthy control group,and CD4^(+)/CD8^(+) was lower than that in the healthy control group(P<0.01);the CD8^(+),CD4^(+)CD25^(+)CD127low/CD4^(+)T cells and CD127low/CD127high of collection product in the collection product group were higher than those in the healthy control group,while CD4^(+)/CD8^(+) was lower than that in the healthy control group(P<0.05).Conclusion In order to ensure the quality of CAR-T cell collection and the safety of donors,there must be good blood routine indexes and blood calcium level before collection,and a good immune balance state should be maintained.
作者 于倩倩 孙俊波 王会平 陆应玉 陶千山 YU Qianqian;SUN Junbo;WANG Huiping;LU Yingyu;TAO Qianshan(the Second Hospital of Anhui Medical University,Hefei 230601,China)
出处 《临床医学研究与实践》 2021年第32期27-30,共4页 Clinical Research and Practice
基金 安徽省高校自然科学基金重点项目(No.KJ2019A0254)。
关键词 CAR-T细胞疗法 T亚群 TREG细胞 CAR-T cell therapy T cell subset Treg cell
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