姜黄素提取物对OSF模型大鼠TGF-β_(1)、IL-1和Bcl-2的影响及相关机制探讨

Effect of curcumin extract on TGF-β_(1),IL-1 and Bcl-2 in OSF model rats and its related mechanism

目的探讨姜黄素提取物对口腔黏膜下纤维化(OSF)模型大鼠转化生长因子β_(1)(TGF-β_(1))、白介素1(IL-1)和B淋巴细胞瘤2(Bcl-2)的影响及相关机制。方法2021年3—6月于湖南中医药大学第一附属医院动物研究中心进行实验。选取清洁SPF级大鼠60只,随机数字表法选取15只为正常对照组,其余大鼠建立OSF模型,成功42只,随机分成模型组、阳性对照组和姜黄素提取物组,各14只。阳性对照组大鼠皮下注射曲安奈德,姜黄素提取物组大鼠给予姜黄素提取物灌胃,正常对照组和模型组大鼠给予等体积生理盐水灌胃。干预8周后,观察各组大鼠口腔黏膜病理组织学变化,比较开口度情况,检测TGF-β_(1)、IL-1和Bcl-2蛋白表达量及Wnt信号通路因子表达量。结果与模型组比较,阳性对照组、姜黄素提取物组大鼠实验第1、3个月张口度增大(P<0.05),且姜黄素提取物组大于阳性对照组(P<0.05)。与模型组比较,阳性对照组、姜黄素提取物组血清TGF-β_(1)、IL-1、Bcl-2蛋白表达量降低(P<0.05),且姜黄素提取物组低于阳性对照组(P<0.05)。与模型组比较,阳性对照组、姜黄素提取物组β-catenin、APC、Bcl-2 mRNA表达量均降低(P<0.05),且姜黄素提取物组低于阳性对照组(P<0.05)。结论姜黄素提取物可能通过作用于Wnt信号通路,调控β-catenin、APC、Bcl-2 mRNA表达量,降低TGF-β_(1)、IL-1、Bcl-2水平,改善纤维化情况。

Objective To explore the effect of curcumin extract on transforming growth factorβ-1(TGF-β_(1)),interleukin 1(IL-1)and B lymphocyte tumor-2(Bcl-2)and related mechanisms of oral submucosal fibrosis(OSF)model rats.Methods Experiments will be conducted in the Animal Research Center of the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine from March to June 2021.A total of 60 clean SPF rats were selected,15 rats were selected as the normal control group by the random number table method,and OSF models were established for the rest of the rats,and then randomly divided into a model group,a positive control group and a curcumin extract group,each with 14 rats.The rats in the positive control group were injected with triamcinolone acetonide subcutaneously,the rats in the curcumin extract group were given the curcumin extract by gavage,and the rats in the normal control group and the model group were given the same volume of normal saline by gavage.After 8 weeks of intervention,the histopathological changes of the oral mucosa of each group were observed,the degree of opening was compared,and the expression of TGF-β_(1),IL-1 and Bcl-2 protein and the expression of Wnt signaling pathway factors were detected.Results Compared with the model group,the mouth opening of rats in the positive control group and the curcumin extract group increased in the first and third months of the experiment(P<0.05),and the curcumin extract group was greater than the positive control group(P<0.05).Compared with the model group,the serum TGF-β_(1),IL-1,and Bcl-2 protein expression in the positive control group and curcumin extract group decreased(P<0.05),and the curcumin extract group was lower than the positive control group(P<0.05).Compared with the model group,the expressions ofβcatenin,APC,and Bcl-2 mRNA in the positive control group and the curcumin extract group were all decreased(P<0.05),and the curcumin extract group was lower than the positive control group(P<0.05).Conclusion Curcumin extract may regulate the expression ofβ-catenin,APC,Bcl-2 mRNA,reduce the levels of TGF-β_(1),IL-1,and Bcl-2,and improve fibrosis by acting on the Wnt signaling pathway.