青稞抗旱基因HVUL1H24632.2的功能验证

Functional Verification of Drought Resistance Gene HVul1H24632.2 of Highland Barley

本研究选择水稻为受体材料,通过农杆菌EHA105侵染水稻愈伤组织的方法转化水稻,对转基因水稻和非转基因水稻模拟干旱处理,然后对逆境处理下的转基因和非转基因材料叶片进行POD,SOD,Pro的检测。结果显示,对照组样品逆境处理下含量明显上升,对干旱胁迫反应敏感,而转基因材料有一定程度上升,上升速度较慢,对干旱胁迫反应不敏感,说明转基因水稻具有一定的抗逆能力。

This study selected rice as receptor material. Rice was transformed by Agrobacterium EHA105 infecting callus of rice. Drought were simulated for transgenic rice and non-transgenic rice. Then,POD,SOD and Pro were detected in transgenic and non-transgenic rice leaves under stress treatment. The content of POD,SOD and Pro was statistically analyzed,The results showed that the contents of POD,SOD and Pro in CK group increased significantly under stress treatment,which showed non-transgenic rice was sensitive to drought stress response. While the contents of POD,SOD and Pro in transgenic materials increased to a certain extent,but the rise rate was slower,which showed that the transgenic materials was not sensitive to drought stress response. It shows that transgenic rice has a certain ability of stress resistance.