益生菌联合美沙拉嗪对小鼠溃疡性结肠炎模型外周血T淋巴细胞亚群及炎症细胞因子影响的研究

Effects of probiotics combined with mesalazine on peripheral blood T lymphocyte subsets and inflammatory cytokines in a mouse model of ulcerative colitis

目的研究益生菌联合美沙拉嗪对小鼠溃疡性结肠炎模型的治疗效果,并探讨联合治疗对溃疡性结肠炎模型外周血T淋巴细胞亚群及炎症细胞因子的影响。方法45只C57BL/6小鼠根据随机数字表法分为3组:正常对照组、模型对照组和联合治疗组,每组各15只。模型对照组和联合治疗组通过饮用葡聚糖硫酸钠建立溃疡性结肠炎模型,联合治疗组小鼠给予益生菌和美沙拉嗪灌胃治疗,正常对照组和模型对照组给予0.9%氯化钠溶液灌胃。比较3组小鼠摄食量、饮水量、尿量、体重、结肠长度、脾脏指数、DAI评分、结肠组织肉眼评分以及外周血T淋巴细胞亚群比例和血清炎症因子[肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-6、IL-17A、IL-10]含量。结果模型对照组小鼠摄食量、饮水量、尿量、体重、结肠长度、外周血CD4^(+)细胞百分率、CD4^(+)/CD8^(+)比例以及血清IL-10含量均低于正常对照组小鼠,脾脏指数、DAI评分、肉眼评分、外周血CD8^(+)细胞百分率以及血清TNF-α、IL-6、IL-17A均高于正常对照组小鼠,差异均有统计学意义(P<0.05)。治疗后,联合治疗组小鼠摄食量、饮水量、尿量、体重、结肠长度、外周血CD4^(+)细胞百分率、CD4^(+)/CD8^(+)比例以及血清IL-10含量均高于模型对照组小鼠,差异均有统计学意义(P<0.05),而联合治疗组小鼠脾脏指数、DAI评分、结肠组织肉眼评分、外周血CD8^(+)细胞百分率以及血清TNF-α、IL-6、IL-17A均低于模型对照组小鼠,差异均有统计学意义(P<0.05);3组小鼠外周血CD3^(+)细胞百分比率差异无统计学意义(P>0.05)。结论益生菌联合美沙拉嗪治疗可以通过调节溃疡性结肠炎小鼠T淋巴细胞亚群而发挥抗炎和治疗作用。

Objective To study the therapeutic effect of probiotics combined with mesalazine on mouse ulcerative colitis model,and to explore the effects of probiotics combined with mesalazine on peripheral blood T lymphocyte subsets and inflammatory cytokines in a mouse model of ulcerative colitis.Methods Forty-five C57BL/6 mice were divided into 3 groups according to the random number table method:normal control group,model control group and combined treatment group,15 mice in each group.The model control group and the combined treatment group established ulcerative colitis models by drinking dextran sodium sulfate,and the mice in the combined treatment group were also given probiotics and mesalazine intragastrically,and the normal control group and the model control group were given intragastric normal saline.The food intake,water intake,urine output,body weight,colon length,spleen index,DAI score,macroscopic score of colon tissue,and the proportion of peripheral blood T lymphocyte subsets and serum inflammatory factor[tumor necrosis factorα(TNF-α),interleukin(IL)-6,IL-17A,IL-10]levels were compared among the three groups of mice.Results The food intake,water intake,urine output,body weight,colon length,peripheral blood CD4^(+)cell percentage,CD4^(+)/CD8^(+)ratio and serum IL-10 content of mice in the model control group were all lower than those in the normal control group,the differences were statistically significant(P<0.05);while the spleen index,DAI score,macroscopic score of colon tissue,percentage of peripheral blood CD8^(+)cells and serum TNF-α,IL-6,IL-17A in the model control group were higher than those in the normal control group,the differences were statistically significant(P<0.05).After treatment,the food intake,water intake,urine output,body weight,colon length,peripheral blood CD4^(+)cell percentage,CD4^(+)/CD8^(+)ratio and serum IL-10 content of mice in the combined treatment group were all higher than those in the model control group,the differences were statistically significant(P<0.05);while the spleen index,DAI score,macroscopic score of colon tissue,percentage of peripheral blood CD8^(+)cells and serum TNF-α,IL-6,IL-17A in the combined treatment group were lower than those in the model control group,the differences were statistically significant(P<0.05);there was no significant difference in the percentage of CD3^(+)cells in peripheral blood between the three groups,the differences were statistically significant(P>0.05).Conclusion Probiotics combined with mesalazine treatment can exert anti-inflammatory and therapeutic effects by regulating the T lymphocyte subsets of mice with ulcerative colitis.