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基于重水拉曼技术评价口腔抑菌剂对粪肠球菌抑菌效能的研究

Efficacy Assessment of Clinical Disinfectants for Enterococcus Faecalis via Single-cell Raman Microspectroscopy
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摘要 目的:开发有效且对患者副作用小的口腔抑菌剂配方,系统评价过氧化氢(hydrogen peroxide,H_(2)O_(2))、次氯酸钠(Sodium hypochlorite,NaClO)对粪肠球菌(Enterococcus faecalis ATCC29212)的抑菌效能。方法:分别采用肉汤稀释法和重水拉曼技术(D_(2)O-labeled single-cell Raman micro-spectroscopy),通过最小抑菌浓度(minimum inhibitory concentration,MIC)和最低抑制代谢浓度(minimum inhibitory concentration based on metabolic activity,MIC-MA)定量评价了抑菌剂对粪肠球菌的生长和代谢抑制作用。结果:对于过氧化氢和次氯酸钠,MIC分别为110 mg/L和0.45 g/L,尽管生长完全停止,但细菌细胞的代谢活性在8 h平均降低了71%、70%,显示出一种“非生长但代谢活跃”(NGMA)的状态,这种状态可能导致潜在的难治性反复感染。而MIC-MA分别为220 mg/L和0.9 g/L时,所有细胞的代谢活动在暴露8 h后完全停止。此外,NaClO+H_(2)O_(2)的组合使用优于单独使用次氯酸钠、过氧化氢。结论:MIC-MA有利于严格评估抗细菌疗效,NaClO+H_(2)O_(2)可作为细菌病原体更有效的抑菌剂方案。 Objective:To evaluate the susceptibility of enterococcus faecalis(Ef)to clinical disinfectants for the development of effective yet patient-friendly disinfectant formulations.Methods:The growth and metabolism-inhibiting effects of intracanal disinfectants via minimum inhibitory concentration(MIC)and minimum inhibitory concentration were quantitatively assessed based on metabolic activity(MIC-MA),using broth dilution test and D_(2)O-probed Single-Cell Raman Spectra(SCRS)respectively.Results:For hydrogen peroxide(H_(2)O_(2))and sodium hypochlorite(NaClO),at their MIC of 110 mg/L and 0.45 g/L respectively,despite complete halt of growth,metabolic activity of bacterial cells was reduced averagely for 71%and 70%at 8 hours,revealing a"nongrowing but metabolically active"(NGMA)state that may underlie potential refractory infections.In contrast,at their MIC-MA of 220 mg/L and 0.9 g/L respectively,metabolic activity of all cells was completely halted throughout 8 hours'exposure.Moreover,the combination usage of NaClO+H_(2)O_(2) outperformed the solo usage of NaClO or H_(2)O_(2).Conclusion:MIC-MA is advantageous in critically assessing antibacterial efficacy,and NaClO+H_(2)O_(2) can potentially serve as a more efficient disinfectant formula for bacterial pathogens.
作者 刘育含 马玉莹 张利娟 李修珍 杨加震 牛玉芬 孙雁斐 曾飞 杨芳 LIU Yuhan;MA Yuying;ZHANG Lijuan;LI Xiuzhen;YANG Jiazhen;NIU Yufen;SUN Yanfei;ZENG Fei;YANG Fang(School of Stomatology, Qingdao University, Qingdao 266000, China;Stomatology Center, Qingdao Municipal Hospital, Qingdao 266071, China;Department of Stomatology, Women & Children's Health Care Hospital of Linyi, Linyi 276002, China;School of Stomatology, Dalian Medical University, Dalian 116044, China)
出处 《口腔医学研究》 CAS CSCD 北大核心 2021年第11期989-993,共5页 Journal of Oral Science Research
基金 国家自然科学基金(编号:81670979,31300424)。
关键词 拉曼显微光谱 重水 粪肠球菌 单细胞技术 临床抑菌剂 raman micro-spectroscopy heavy water Enterococcus faecalis single-cell technology clinical disinfectants
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