人脐带间充质干细胞促进血小板血浆凝固的机制

Mechanism of human umbilical cord mesenchymal stem cells to promote platelet plasma coagulation

背景:随着间充质干细胞临床应用研究的深入,间充质干细胞的临床使用方法逐渐发生变化,为增强干细胞在局部组织发挥作用,临床中一般选择干细胞联合凝胶局部使用,而自体富血小板血浆是一种理想的凝胶替代材料。目的:探讨人脐带间充质干细胞促进血小板血浆凝固的机制。方法:①将人脐带间充质干细胞与抗凝血浆混匀,添加氯化钙刺激凝血,记录血浆凝血时间,分析人脐带间充质干细胞数量对血浆凝固时间的影响;②通过流式分析CD142凝血因子在人脐带间充质干细胞内的表达情况,使用CD142抗体预处理人脐带间充质干细胞,验证CD142在人脐带间充质干细胞促进凝血过程中是否发挥作用;③查找数据库分析人脐带间充质干细胞中CD142的表达调控模式,并通过WGCNA方法以及文献语义分析CD142在人脐带间充质干细胞中高表达的机制。结果与结论:①人脐带间充质干细胞通过表达CD142加速血小板血浆凝固,人脐带间充质干细胞中CD142表达随细胞代次增加而增加;②CD142表达上调是人脐带间充质干细胞自分泌的结果;③CD142能够影响人脐带间充质干细胞趋化性以及定植能力。

BACKGROUND:With the in-depth study of clinical application of mesenchymal stem cell,the clinical use of mesenchymal stem cell has gradually changed.To enhance the role of mesenchymal stem cell in local tissues,stem cell is generally used in combination with local injection of gel,and autologous platelet-rich plasma is an ideal gel replacement material.OBJECTIVE:To explore the mechanism of human umbilical cord mesenchymal stem cells to promote platelet plasma coagulation.METHODS:(1)Stem cell was mixed with anticoagulated plasma.Calcium chloride was used to stimulate coagulation.Clotting time was recorded.The effect of the number of human umbilical cord mesenchymal stem cells on the clotting time of plasma was analyzed.(2)CD142 expression in stem cell was analyzed by flow cytometry.CD142 antibody was used to pretreat human umbilical cord mesenchymal stem cells and to ensure if CD142 was one of the most important factors to improve plasma coagulation.(3)CD142 expressing model and regulation model were analyzed by checking on database.WGCNA and text mining were used to achieve the result why CD142 gene expression level was high in human umbilical cord mesenchymal stem cells.RESULTS AND CONCLUSION:(1)Platelet plasma coagulation could be accelerated by CD142 expression of human umbilical cord mesenchymal stem cells.CD142 expression was enhanced as the increase in mesenchymal stem cell passage.(2)CD142 upregulation was a result of mesenchymal stem cell autocrine.(3)CD142 could affect the ability of cell chemotaxis and colonization in human umbilical cord mesenchymal stem cells.