花生乙酰辅酶A羧化酶家族基因在不同胁迫下的响应模式分析

Response expression patterns analysis to different stresses of acetylCo A carboxylase family genes in peanut

乙酰辅酶A羧化酶(acetyl-CoA carboxylase, ACC)在植物中催化乙酰辅酶A生成丙二酰辅酶A,是脂肪酸合成的关键酶。为系统分析花生乙酰辅酶A羧化酶基因家族,对28个AhACC基因进行了生物信息学分析。结果表明, 28个AhACC基因分别编码异质型ACC的4个亚基BC、BCCP、α-CT、β-CT和同质型ACC,基因个数为7、13、4、2和2个,不均匀分布在15条染色体上,只有一个具有跨膜结构域。同质型AhACC外显子个数最多为32个, AhaccD外显子个数最少为1个。聚类分析表明, AhACC与大豆、苜蓿亲缘关系较近,编码花生BCCP亚基的基因数目最多。根据转录组数据发现, AhACC多存在于叶片和种子发育早期,在不同组织中有不同表达模式,另外AhACC在根中主要响应ABA胁迫,同质型AhACC对低温和盐胁迫有重要作用。从转录组数据库中挑选8个差异表达基因进行qRT-PCR验证,其表达趋势与测序结果相一致。8个差异表达的基因在ABA处理24 h后表达量全部上调;arahy.15M9SP和arahy.Y8HZNS在NaCl处理后表达量下调,其余6个表达量上调。

Acetyl-CoA carboxylase(ACC), the key enzyme in fatty acid synthesis, catalyzes acetyl-CoA to malonyl-Co A in plants. To systematically analyze the gene family of ACC in peanut, 28 Ah ACC genes were analyzed by bioinformatics. The results showed that 28 AhACC genes encode four subunits of heterogeneous ACC(BC, BCCP, α-CT, β-CT) and homogeneous ACC respectively, and the number of genes is 7, 13, 4, 2 and 2. They were unevenly distributed on 15 chromosomes, and only one had transmembrane domain. The maximum number of homomorphic AhACC exons was 32, and the minimum number of exons of AhaccD was 1. Cluster analysis showed that AhACC was closely related to soybean and alfalfa, and the number of genes encoding BCCP subunit in peanut was the largest. According to the transcriptome data, AhACC mostly existed in the early stage of leaf and seed development, and had different expression patterns in different tissues. In addition, AhACC mainly responded to ABA stress in roots, and homomorphic Ah ACC played an important role in low temperature and salt stress. Subsequently, qRT-PCR of eight differentially expressed genes randomly selected from RNA-Seq database was carried out, and the expression pattern was consistent with the results of RNA-sequencing. The expression level of the eight differentially expressed genes was up-regulated after 24 h ABA stress;the expression level of arahy.15 M9 SP and arahy.Y8 HZNS was down-regulated after NaCl stress, whereas the other six genes were up-expressed.