过表达AtMIR160对亚麻芥(Camelina sativa)根尖发育的影响

Overexpression AtMIR160 affects root tips development in Camelina sativa

亚麻芥(Camelina sativa)作为重要的油料作物,其研究获得越来越多的关注。本实验通过Lugol淀粉粒染色观察了亚麻芥与拟南芥的根尖结构,并构建了亚麻芥的AtMIR160过表达株系,探索过表达AtMIR160对亚麻芥根尖发育的影响。结果显示:与拟南芥根尖静止中心和柱干细胞中无淀粉粒分布不同,在亚麻芥根尖可能是静止中心和柱干细胞的位置有淀粉粒分布。进一步在亚麻芥中过表达AtMIR160,qRT-PCR分析显示亚麻芥AtMIR160过表达株系中其同源靶基因CsARF10和CsARF16的表达量显著降低,AtMIR160过表达株系呈现根冠细胞排列紊乱和淀粉粒减少的表型。以上结果说明亚麻芥和拟南芥根尖淀粉粒的分布存在差异,拟南芥MIR160能通过下调亚麻芥同源靶基因影响其根冠发育。

As an important oil crop, more and more attention was obtained in Camelina sativa-related research. In this paper, Lugol starch grain staining was used to observe the structure of root tips in C. sativa and Arabidopsis thaliana, and the AtMIR160 overexpression lines of C. sativa were constructed to explore the effects of AtMIR160 on the development of root tips in C. sativa. The results showed that starch grains were distributed in the possible location of the quiescent center and stem cells of the root tips in C. sativa. This is different from the quiescent center and stem cells of A. thaliana root tips, which have non-starch grain distribution. Furthermore, when AtMIR160 was overexpressed in C. sativa, qRT-PCR analysis showed that the expression of the homologous target genes CsARF10 and CsARF16 was significantly reduced in the AtMIR160 overexpression lines of C. sativa. The transgenic lines showed a phenotype of disordered arrangement of root cap cells and reduced starch grains. The above results indicate that there are differences in the distribution of starch grains in the root tip of C. sativa and A. thaliana, and AtMIR160 can affect the root cap development of C. sativa by down-regulating its homologous target genes.