烯效唑对低温胁迫下绿豆初花期碳代谢、抗氧化系统及产量的影响

Effect of uniconazole on carbon metabolism, antioxidant system and yield of mung bean at initial flowering stage under low temperature stress

以绿豆‘绿丰5号’为材料,研究15℃低温胁迫下叶面喷施50 mg·L^(-1)烯效唑(S3307)对初花期叶片碳代谢、抗氧化酶活性及产量的影响。结果表明:外源喷施S3307能够显著增强低温胁迫下叶片过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)、蔗糖磷酸合成酶(SPS)和α-淀粉酶活性,同时降低过氧化氢酶(CAT)和蔗糖合成酶(SS)活性,而对超氧化物歧化酶(SOD)、中性转化酶(NI)和酸性转化酶(AI)活性影响不显著;促进可溶性糖、蔗糖和淀粉积累,降低果糖含量;抑制丙二醛(MDA)含量增加,显著降低相对电导率(REC)、过氧化氢(H2O2)水平和超氧阴离子(O_(2)^(-)·)产生速率。此外,叶面喷施S3307对低温胁迫下的产量损失具缓解作用,但差异不显著。综上所述,外源喷施S3307通过提高叶片碳代谢能力和抗氧化系统活性,减轻了低温对绿豆造成的伤害,从而在一定程度上缓解了低温胁迫下的产量损失。

This study was conducted with mung bean ’Lüfeng5’ to investigate the effect of foliar spraying 50 mg·L^(-1) uniconazole(S3307) on carbon metabolism, antioxidant enzyme activity, and yield at the initial flowering stage under low temperature stress of 15℃. The results showed that exogenous spraying of S3307 dramatically enhanced the activities of peroxidase(POD), ascorbate peroxidase(APX), glutathione reductase(GR), sucrose phosphate synthase(SPS), and α-amylase, diminished the activities of catalase(CAT) and sucrose synthase(SS), but had no significant effect on the activities of superoxide dismutase(SOD), neutral invertase(NI), and acid invertase(AI) in leaves under low temperature stress. Application of S3307 promoted the accumulation of soluble sugar, sucrose and starch, decreased the content of fructose, inhibited the increase of malondialdehyde(MDA) content, and considerably reduced the relative electrical conductivity(REC), hydrogen peroxide(H2O2) level, and superoxide anion(O_(2)^(-)·) production rate. Furthermore, foliar spraying S3307 could reduce the yield loss under low temperature stress, but there was no significant difference compared to the low temperature stress alone. In conclusion, the exogenous application of S3307 reduced the damage caused by low temperature to mung beans by improving the ability of carbon metabolism and the activity of the antioxidant system in leaves, thus alleviating the yield loss under low temperature stress.