温度对笼目海带配子体生长发育的影响

Effects of Temperature on Growth and Development of Gametophytes in Seaweed Kjellmaniella crassifolia

将笼目海带雌、雄配子体按2∶1的比例混合后,取10mL悬浊液加入到直径12cm的培养皿中,置于5、8、12、15、20、25℃下培养,光周期10L∶14D,光照度1000lx,观察其生长发育,计算萌发率(包括卵囊形成率、排卵率、幼苗形成率),探讨水温对笼目海带配子体生长及发育的影响。试验结果表明,8~12℃下,80%~90%配子体进入了生殖生长,50%以上为多细胞幼苗。在5、15℃下,70%~80%配子体处于营养生长,虽有20%~30%配子体进入生殖生长,但明显滞后于8~12℃温度组。20℃和25℃下所有配子体维持在营养生长状态。因此建议笼目海带配子体育苗的温度控制在8~12℃。

Ten mL of suspension fluid containing female and male gametophytes of seaweed Kjellmaniella crassifolia with 2♀∶1♂ratio were cultivated in a petri dish with a diameter of 12 cm at water temperature of 5,8,12,15,20,and 25℃,photoperiod of 10L∶14D,and light intensity of 1000 lx to explore the effect of water temperature on the gametophyte growth and development via determination of germination rate including oocystogenesis rate,ovulation rate,and seedling formation rate.It was found that 80%-90%of the gametophytes was developed into juveniles at 8-12℃,with 50%of the seedlings.At water temperature of 5℃and 15℃,however,70%-80%of the gametophytes were still kept at the vegetative stage,although a small proportion of gametophytes(20%-30%)entered into the developmental state,significantly retared behind the group teated at 8-12℃.Those gametophytes were all remained at vegetative stage at 20℃and 25℃.It is concluded that 8-12℃is the optimum condition for the development of gametophytes.