星形胶质细胞瘤中神经胶质细胞成熟因子β和Ki-67表达的相关性及其在预后评估中的意义

Association of GMF-βexpression with Ki-67 and its significance in the prognostic evaluation of astrocytoma

目的了解星形胶质细胞瘤中神经胶质细胞成熟因子β(glia maturation factor-β,GMF-β)和Ki-67表达的相关性,并探讨GMF-β/Ki-67联合检测应用于胶质瘤预后评估的意义。方法收集陆军军医大学(原第三军医大学)西南医院2006—2009年胶质瘤病例140例(WHOⅡ~Ⅳ级)的组织样本、临床病理信息和3年随访资料;免疫组织化学单标、双标染色检测肿瘤细胞GMF-β和Ki-67的表达,统计学分析两者的相关性以及对患者预后的影响。结果星形胶质细胞瘤GMF-β表达水平与肿瘤级别、Ki-67表达均相关(均P<0.05);Kaplan-Meier生存率分析显示,GMF-β、Ki-67表达水平分别与患者3年生存率呈负相关(均P<0.01)。进一步分析证实两者共同影响生存期长短,呈现GMF-β低表达+Ki-67低表达组>GMF-β高表达+Ki-67低表达组>GMF-β低表达+Ki-67高表达组>GMF-β高表达+Ki-67高表达组的3年生存率趋势,组间差异均有统计学意义(分别P<0.05,P<0.01)。结论星形胶质细胞瘤GMF-β和Ki-67的表达水平正相关,GMF-β/Ki-67联合检测可应用于胶质瘤的临床预后评估。

Objective To clarify the correlation of the expression of glia maturation factor-β(GMF-β)with Ki-67 in astrocytoma,and to investigate the prognostic implications of combined detection of GMF-βand Ki-67.Methods One hundred and forty human astrocytoma samples(WHOⅡ-Ⅳgrade)were collected at Southwest Hospital,Army Medical University(the Third Military Medical University),China from 2006 to 2009.Clinicopathological information and 3-year follow-up data were collected.Expression of GMF-βand Ki-67 was detected by single and double immunohistochemical staining,then the association of GMF-βexpression with Ki-67 and its significance in prognostic evaluation of astrocytoma were statistically analyzed.Results GMF-βexpression in astrocytoma cells was correlated to both tumor grade and Ki-67(both P<0.05);Kaplan-Meier survival analysis showed that GMF-βand Ki-67 expression were negatively correlated to the 3 year-survival rates,respectively(both P<0.01).Further analysis demonstrated that the two factors were co-influenced on survival,showing a trend of"GMF-βlow Ki-67low>GMF-βhigh Ki-67low>GMF-βlow Ki-67high>GMF-βhigh Ki-67high"in 3-year survival rate with significant intergroup differences(P<0.05,P<0.01).Conclusions GMF-βexpression is positively associated with Ki-67 in astrocytoma.Combined detection of GMF-βand Ki-67 can predict prognosis of patients with glioma.