基于BSA-seq法的纤维衣分相关候选基因的鉴定

Identification of Candidate Genes Associating with Fiber Lint Percentage Using BSA-seq

衣分是影响棉纤维产量的重要指标。本研究以高衣分棉花陆海渐渗系材料MBI7747-14和中棉所45为亲本,构建包含2403个单株的F2分离群体,挑选衣分性状极端单株构建2个混池,采用BSA-seq方法进行衣分相关基因定位分析。结果表明,在D2染色体上得到4个置信指数高于95%的候选区间,4个区间共包含236个基因,总长度为5.47 Mb。在以上基因中,200个基因中含有SNP,190个基因中含有InDel,其中70个基因含有非同义突变位点。通过转录组数据的基因表达模式分析,筛选出19个可能与衣分相关的候选基因。GO功能富集分析表明,19个候选基因集中于NADP+活性、醛糖醇代谢、碳利用和调控细胞发育等功能条目。本研究为进一步研究棉纤维衣分形成的遗传机制奠定了一定的基础。

Lint percentage is an important index affecting cotton fiber yield.In this study,an F2 segregation population containing 2403 individual plants was constructed derived from chromosome introgression line MBI7747-14(Gossypium barbadense x Gossypium hirsutum)with high lint percentage crossing with CCRI45.The BSA-seq was conducted using two bulked samples by pooling individual plants showing higher and lower lint percentage,respectively.As a result,four candidate physical regions showing confidence indices higher than 95%were obtained in a total length of 5.47 Mb on chromosome D2,which contained 236 annotated genes.Out of them,200 genes contain SNPs,190 genes contain Indel,and 70 genes contain non-synonymous mutation sites.Through gene expression pattern analysis of transcriptome data,19 candidate genes possibly associating with lint percentage were identified.GO functional enrichment analysis showed that the 19 candidate genes were enriched in NADP+activity,aldol metabolism,carbon utilization and regulation of cell development.This study laid a foundation for further analyzing the genetic mechanism of cotton fiber lint percentage formation.