miRNA-145负向调控子宫内膜异位症中OCT4的表达

MiRNA-145 Negatively Regulates OCT4 Expression in Endometriosis

目的探讨miRNA-145、OCT4在子宫内膜异位症(EMs)中的表达水平及临床意义。方法收集2018年8月至2020年8月昆明医科大学第二附属医院妇科收治的EMs患者30例(实验组),不孕症(输卵管因素)患者30例(对照组),实验组术中取异位子宫内膜组织,对照组术中取在位子宫内膜组织,QPCR检测异位内膜组织、在位内膜组织中miRNA-145、OCT4的表达,Western印迹法检测组织中OCT4蛋白的表达。结果EMs患者子宫内膜异位组织中较非EMs患者在位内膜组织中miRNA-145表达增高(P<0.01),而OCT4表达下调(P<0.01),2者呈现负相关关系。结论miRNA-145有望成为子宫内膜异位症诊断的潜在标志物。miRNA-145与OCT4在子宫内膜异位症发生、发展中具有一定的作用,这一发现为EMs的发病机制及治疗提供了可能的研究方向及潜在靶点。

Objective To study the expression and clinical significance of miRNA-145 and OCT4 in endometriosis.Methods From August 2018 to August 2020,30 EMs patients(experimental group)and 30 patients with infertility caused by fallopian tube factors(control group)were collected.Ectopic endometrial tissues were collected during operation in experimental group and eutopic endometrial tissues in control group.The expressions of miRNA-145 and OCT4 in ectopic and eutopic endometrial tissues were detected by QPCR,and the expression of OCT4 protein in tissues was detected by Western blot.Results Compared with non-EMs patients,the expression of miRNA-145 in endometriotic tissues increased(P<0.01),while the expression of OCT4 decreased(P<0.01),showing a negative correlation between them.Conclusion miRNA-145 is expected to be a potential marker for diagnosis of endometriosis.miRNA-145 and OCT4 play a certain role in the occurrence and development of endometriosis,and this finding provides a possible research direction and potential target for the pathogenesis and treatment of EMS.