IL-38、IL-29、CKLF-1与寻常型银屑病的相关性分析

Analysis of the association of IL-38,IL-29 and CKLF-1 with psoriasis vulgaris

目的检测寻常型银屑病患者血清中IL-38、IL-29、趋化素样因子1(CKLF-1)的表达水平,与已知银屑病治疗靶点IL-17A水平对比,探讨其与银屑病严重程度的关系,指导临床实践。方法选取寻常型银屑病(PV)患者40例(实验组),行常规药物治疗8周,并以30例同期健康者为对照,采用双抗体夹心ELISA法检测实验组治疗前后及对照组外周血,统计分析外周血中IL-38、IL-29、CKLF-1、IL-17A的表达水平。结果实验组治疗前外周血中IL-38表达水平与治疗后和对照组相比均显著降低(P<0.05)。实验组治疗前外周血中IL-29、IL-17A表达水平与治疗后和对照组相比均明显升高(P<0.05)。实验组治疗前外周血中CKLF-1表达水平与治疗后和对照相比差异无统计学意义(P>0.05)。IL-38与IL-17A水平呈负相关(r=-0.841,P<0.01),IL-38与IL-29水平呈负相关(r=-0.780,P<0.01);IL-29与IL-17A水平呈正相关(r=0.854,P<0.01),CKLF-1与IL-17A水平无显著相关性(P>0.05)。结论寻常型银屑病患者血清中的IL-38作为抑炎因子参与银屑病的发病过程,IL-29作为促炎因子参与银屑病的发病,CKLF-1可能未在血清水平参与银屑病的发病过程。

Objective To examine the levels of serum IL-38,IL-29 and CKLF-1 in patients with psoriasis vulgaris(PV),and explore their association with psoriasis vulgaris through comparing the level of the recognized therapeutic target IL-17A.Methods A total of 40 PV patients were selected as an experiment group,and treated with conventional medications for eight weeks.Meanwhile,30 healthy subjects were selected as a control group.Their peripheral blood samples were measured by ELISA before and after treatment to examine the association of IL-38,IL-29 and CKLF-1 with IL-17A.Results The experiment group presented remarkable decreases in the level of peripheral blood IL-38,compared with the control group before treatment and those after treatment(P<0.05).The experiment group also showed significant increases in the levels of peripheral blood IL-29 and IL-17A,compared with the control group before treatment and those after treatment(P<0.05).The experiment group presented no significant difference in the level of peripheral blood CKLF-1,compared with the control group before treatment and those after treatment(P>0.05).The level of IL-38 in PV patients was negatively associated with IL-17A(r=-0.841,P<0.01),and IL-29(r=-0.780,P<0.01).The level of IL-29 in PV patients was positively associated with IL-17A(r=0.854,P<0.01).However,there was no association between the level of CKLF-1 and IL-17A(P>0.05).Conclusions Serum IL-38 is involved as an anti-inflammatory factor in the pathogenesis of PV,while IL-29 acts as a pro-inflammatory factor.Serum CKLF-1 may not be involved in the onset of PV.