高原鼢鼠发情周期阴道细胞变化特征及血清促性腺激素含量动态

Characteristics of vaginal cells and dynamics of serum gonadotropin content in estrus cycle of plateau zokor(Eospalax baileyi)

野生动物繁殖调控是动物生态学研究的核心问题之一,其目的是通过研究调控动物个体及种群繁殖因素,揭示物种繁殖调控和生存规律(孙儒泳等,1997;Zenuto et al.,2001)。一般而言,雌性生殖细胞生殖能力的获得需经历卵泡发育和卵子发生两个过程,而这两个相互依存的过程皆在发情周期完成。发情周期的启动为雌雄配子相互结合及下一代个体发育奠定基础(张嘉保和田见晖,2011)。

In order to determine the change characteristics of vaginal cells in the estrus cycle and the duration of the estrus cycle,vaginal smears and HE staining methods were used to observe and count the types,morphological changes,and proportions of vaginal cells in plateau zokor(Eospalax baileyi).The hormone secretion dynamics of follicle-stimulating hormone(FSH)and luteinizing hormone(LH)in the serum of plateau zokor in the estrus cycle were determined by ELISA.The results showed that the main vaginal cells of plateau zokor were leukocytes,nuclear epithelial cells,incompletely cornified epithelial cells,and complete cornified epithelial cells.The proportion of nuclear epithelial cells in the proestrus was significantly higher than that during the other three periods(48.4±3.09)%(n=12,P<0.05).The proportion of complete cornified epithelial cells in the oestrus was the highest and significantly higher than that in the other three periods(59.73±7.59)%(n=15,P<0.05).The proportion of leukocytes in the metestrus and dioestrum was significantly higher than that in the proestrus and oestrus(P<0.05).The expression level of LH was the highest in the metestrus(4.7095±1.0940)ng/mL(n=6,P<0.05),which showed an increasing trend in proestrus and oestrus,and a decreasing trend in the metestrus and dioestrum.There was no significant difference in the expression level of FSH among the four periods(P>0.05);the estrus cycle duration of plateau zokor was 16 to 19 d,the characteristics of vaginal cell types in different stages of the estrus cycle were obvious,and the relative number was statistically significant.The study provided a basis for vaginal smear identification in the estrous cycle and reproductive physiology study of plateau zokor.