茸桂补肾口服液中补骨脂素和异补骨脂素含量测定

Determination of Psoralen and Isopsoralen in RongGui BuShen Oral Liquid

目的建立茸桂补肾口服液中补骨脂素和异补骨脂素含量测定的方法。方法采用高效液相色谱法,色谱柱为SunFire^(TM)C_(18)column,流动相为乙腈-水(32∶68),流速为1.0 mL·min^(-1),检测波长246 nm。结果补骨脂素进样量在44.15~1104.00 ng范围内与峰面积呈良好的线性关系,相关系数r=0.9999,平均回收率为96.4%,RSD为1.6%;异补骨脂素进样量在34.35~859.00 ng范围内与峰面积呈良好的线性关系,相关系数r=0.9999,平均回收率为94.5%,RSD为1.7%。结论本实验建立的方法具有专属、简便、准确、分离度好的特点,增强了茸桂补肾口服液的质量可控性。

OBJECTIVE To establish a high performance liquid chromatography(HPLC)method for the determination of psoralen and isopsoralen in RongGui BuShen Oral Liquid.METHODS By HPLC,A SunFire^(TM)C_(18)column was used,with mobile phase of acetonitrile-water(32∶68).The flow rate was 1.0 mL·min^(-1) and the detective wavelength was 246 nm.RESULTS The linear range of psoralen was 44.15-1104.00 ng,r=0.9999,the average recovery was 96.4%,with RSD of 1.6%.The linear range of isopsoralen was 34.35-859.00 ng,r=0.9999,the average recovery was 94.5%,with RSD of 1.7%.CONCLUSION The method is specific,simple,accurate and good separable degree.The quality controllability of RongGui Bushen Oral Liquid is enhanced.