摘要
通过采用Griess和酶联免疫吸附测定法检测沙棘黄酮对脂多糖(LPS)诱导小鼠腹腔巨噬细胞(Raw264.7)的细胞吞噬能力、一氧化氮(NO)及炎性因子的影响。分析发现,沙棘黄酮在50~250µg/mL能够显著增加细胞吞噬能力且显著抑制Raw264.7细胞内NO、白细胞介素6(IL-6)、细胞肿瘤坏死因子-α(TNF-α)和环氧化酶-2(COX-2)的产生,并均呈剂量依赖效应。结果表明,沙棘黄酮可通过调控细胞因子和炎性因子分泌而抑制炎症反应。此外,比较了沙棘黄酮与抗坏血酸(VC)的羟自由基、DPPH自由基、ABTS自由基清除能力和总抗氧化(FRAP)差异。结果表明,沙棘黄酮清除DPPH自由基、ABTS自由基的能力和VC相当(IC50值分别为9.014μg/mL、136.86μg/mL),当浓度为80μg/mL时沙棘黄酮的羟自由基清除能力、总还原能力和FRAP分别为59.35%、1.02和2.36mmoL FeSO4/L,说明其具有良好的抗氧化能力。
Griess and enzyme-linked immunosorbent assay were used to detect the effects of sea buckthorn flavonoids on lipopolysaccharide(LPS)-induced mouse peritoneal macrophages(Raw264.7)phagocytic capacity,nitric oxide(NO)and inflammatory factors.Analysis illustrated that sea buckthorn flavonoids at 50-250µg/mL can significantly increase the phagocytic capacity of cells and significantly inhibit the intracellular NO,interleukin 6(IL-6),tumor necrosis factor-α(TNF-α)and Raw264.7 cells.The production of cyclooxygenase-2(COX-2)shows a dose-dependent effect.It showed that sea buckthorn flavonoids can inhibit inflammation by regulating the secretion of cytokines and inflammatory factors.In addition,the differences in hydroxyl free radicals,DPPH free radicals,ABTS free radical scavenging ability and total antioxidant(FRAP)of sea buckthorn flavonoids and ascorbic acid(Vc)were compared.The results showed that the ability of sea buckthorn flavonoids to scavenge DPPH free radicals and ABTS free radicals was equivalent to Vc(IC50 values were 9.014μg/mL,136.86μg/mL,respectively).When the concentration was 80μg/mL,the hydroxyl radical scavenging ability and total reduction capacity and FRAP were 59.35%,1.02and 2.36mmoL FeSO4/L,respectively,indicating that it has good antioxidant capacity.
作者
付依依
王永霞
李月
张笑莹
苑鹏
夏凯
谭志超
FU Yiyi;WANG Yongxia;LI Yue;ZHANG Xiaoying;YUAN Peng;XIA Kai;TAN Zhichao(College of Life Science and Food Engineering,Hebei University of Engineering,Handan 056038;China National Research Institute of Food&Fermentation Industries Co.,Ltd.,Beijing 100015;Zhangbei Baodekang Food Co.,Ltd.,Zhangjiakou 076450)
出处
《中国食品添加剂》
CAS
北大核心
2021年第10期67-74,共8页
China Food Additives
基金
河北省重点研发计划项目(19223002D)。
关键词
沙棘黄酮
小鼠腹腔巨噬细胞
脂多糖
抗炎
抗氧化
sea buckthorn flavonoids
antioxidant
mouse macrophages
lipopolysaccharide
anti-inflammatory