烟草根结线虫病防治药剂对根际土壤细菌群落结构的影响

Effects of control agents against tobacco root-knot nematode disease on bacterial community structure in rhizosphere soil of tobacco plants

为探究烟草根结线虫病不同防治药剂对根际土壤中细菌群落结构的影响,采用Illumina MiSeq高通量测序技术,分别对阿维菌素、枯草芽孢杆菌、淡紫拟青霉、荧光假单胞杆菌、哈茨木霉、阿维·丁硫等药剂防治烟草根结线虫处理后的烟株根际土壤中细菌群落结构变化情况进行了检测。结果表明,与对照组(CK)相比,化学药剂显著降低了细菌群落的物种丰富度和多样性以及群落结构的稳定性;生防菌剂显著提高了细菌群落的物种丰富度和多样性。在门水平上,变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)和酸杆菌门(Acidobacteria)为共有优势菌门,平均相对丰度分别为35.13%、23.12%、13.84%、8.97%;在属水平上,鞘氨醇单胞菌属(Sphingomonas)和芽单胞菌属(Gemmatimonas)为共有优势菌属,平均相对丰度分别为5.67%、3.29%。药剂处理后假节杆菌属(Pseudarthrobacter)、马赛菌属(Massilia)、链霉菌属(Streptomyces)和芽生球菌属(Blastococcus)的相对丰度差异性显著。因此,生防菌剂能够提升根际土壤中细菌群落的物种丰富度和多样性,可调节菌群结构,增加有益菌属的相对丰度,改善土壤生态环境。

In order to explore the effects of different control agents against tobacco root-knot nematode disease on the bacterial community structure in the rhizosphere soil of tobacco plants,the Illumina MiSeq high-throughput sequencing technology was used to detect the changes of the bacterial community structure in the rhizosphere soil of tobacco plants after treated with abamectin,Bacillus subtilis,Paecilomyces lilacinus,Pseudomonas fluorescens,Trichoderma harzianum,and abamectin+carbofuran.The results showed that compared with the control group(CK),the chemical agents significantly reduced the species richness and diversity of the bacterial community,as well as the stability of the community structure.Biocontrol agents significantly increased the species richness and diversity of the bacterial community.At phylum level,Proteobacteria,Actinobacteria,Chloroflexi,and Acidobacteria were shared dominant bacteria phyla with the average relative abundances of 35.13%,23.12%,13.84%,8.97%respectively.At genus level,Sphingomonas and Gemmatimonas were shared dominant bacterial genera with the average relative abundances of 5.67%and 3.29%respectively.The relative abundances of Pseudarthrobacter,Massilia,Streptomyces,and Blastococcus were significantly different after treatment.Therefore,the biocontrol agents can increase the species richness and diversity of the bacterial community in the rhizosphere soil,regulate the community structure,raise the relative abundance of beneficial bacteria,and improve the soil ecological environment.