急性白血病患者细胞质髓过氧化物酶检测结果分析

Results analysis of cytoplasmic myeloperoxidase detection in patients with acute leukemia

目的探讨并比较急性白血病(AL)患者细胞过氧化物酶(POX)染色与细胞质髓过氧化物酶(cMPO)流式细胞术检测的阳性率及阳性积分,评价cMPO流式细胞术的检测能力。方法选取该院60例AL住院患者作为研究对象,分别进行POX染色与cMPO流式细胞术检测,并比较AL患者细胞POX染色与cMPO流式细胞术的阳性率及阳性积分。结果在急性非淋巴细胞白血病(ANLL)中cMPO的阳性率较高,与POX染色的阳性率对比差异无统计学意义(P>0.05);急性淋巴细胞白血病(ALL)的POX染色存在一定的假阳性率。ALL与ANLL的POX染色与cMPO流式细胞术的阳性积分对比,差异无统计学意义(P>0.05)。结论POX染色的准确度低于cMPO流式细胞术,但成本低、仪器依赖性小,建议两种方法联合应用,继而为血液系统疾病的诊断提供客观依据。

Objective To explore and compare the positive rates and positive scores of cellular peroxidase(POX)staining and cytoplasmic myeloperoxidase(cMPO)in the patients with acute leukemia(AL),and to evaluate the detection capability of flow cytometry for detecting cMPO.Methods Sixty inpatients with AL in this hospital were selected as the research subjects,and conduction the POX staining and cMPO detection by flow cytometry.Then the positive rates and positive scores of POX staining and cMPO flow cytometry in the patients with AL were compared.Results In acute non-lymphocytic leukemia(ANLL),the positive rate of cMPO was relatively high,and there was no statistically significant difference compared with the positive rate of POX staining(P>0.05),while there was a certain false positive rate in the POX staining of acute lymphocytic leukemia(ALL).There was no statistically significant difference between the positive score of POX staining and cMPO flow cytometry in ALL and ANLL.Conclusion The accuracy of POX staining is lower than that of cMPO flow cytometry,but its cost is lower and instrument dependence is smaller.It is recommended that the two methods are combined to apply,then to provide an objective basis for the diagnosis of blood system diseases.