高剂量维生素C促进大鼠自体脂肪移植成活

High-dose vitamin C promotes the survival of autologous fat transplantation in rats

背景:脂肪移植常应用于软组织填充、再造、重建及美容等,但脂肪移植的成活率差异较大,寻找提高脂肪移植成活率的方法成为研究重点。目的:探究自体脂肪移植后给予不同剂量维生素C对脂肪移植成活的影响。方法:20只雌性SD大鼠,随机分成低剂量维生素C组、高剂量维生素C组、超高剂量维生素C组及对照组,每组5只。取出各组大鼠腹腔肠系膜脂肪,适当处理后取0.8 g颗粒脂肪移植于背部皮下直径1 cm囊袋中,术后连续7 d前3组分别腹腔注射50,150,1000 mg/d的维生素C溶液1 mL,对照组腹腔注射等量生理盐水。移植后8周将大鼠安乐死,取出移植物,剥除粘连纤维结缔组织后进行大体观察、称移植物留存质量、苏木精-伊红染色、CD34免疫组织化学染色。结果与结论:①3个维生素C组的脂肪质地较对照组柔软,表面有更多的细小血管,脂肪移植物质量均大于对照组,且以高剂量维生素C组效果最明显(P<0.001);②3个维生素C组均可减少囊泡形成,高剂量维生素C组、超高剂量维生素C组在减轻炎性浸润及纤维化上更显著(P<0.05);③高剂量维生素C组、超高剂量维生素C组的毛细血管密度较大,均高于对照组(P<0.001);④结果表明,高剂量维生素C可以提高大鼠自体脂肪的毛细血管密度及留存质量,减轻炎性浸润及纤维化程度,减少囊泡形成。

BACKGROUND:Fat transplantation is often used for soft tissue filling,reconstruction,and beauty treatments.However,the survival rate of fat transplants varies greatly.How to improve the survival rate of fat transplants has become a research focus.OBJECTIVE:To explore the effect of different doses of vitamin C on the survival of fat transplants after autologous fat transplantation.METHODS:Twenty female Sprague-Dawley rats were randomized into a low-,high-,super-high-dose vitamin C groups and control group(n=5 per group).The abdominal mesenteric fat of rats in each group was taken out,and 0.8 g of fat particles was transplanted into a 1 cm diameter subcutaneous sac on the back of each rat after proper treatment.The experimental groups were intraperitoneally injected with 1 mL of vitamin C solution of 50,150,and 1000 mg/d respectively for 7 consecutive days after operation.The control group was intraperitoneally injected with the same amount of normal saline.After 8 weeks,the rats were euthanized.Then the grafts were taken out and evaluated as follows after removal of the adherent fibrous connective tissue:(1)gross observation;(2)retention weight;(3)hematoxylin-eosin staining;(4)immunohistochemical staining of CD34.RESULTS AND CONCLUSION:The texture of fat in the three vitamin C groups was generally softer than that of the control group,and there were more small blood vessels on the surface of the fat graft.The graft retention weight of the vitamin C groups was significantly higher than that of the control group,and the retention weight of the high-dose group was highest(P<0.001).The formation of vesicles in the three vitamin C groups was reduced.High-and super-high-dose vitamin C was more effective in reducing inflammatory infiltration and fibrosis(P<0.05).The capillary densities in the high-and super-high-dose vitamin C groups were higher than that in the control group(P<0.001).To conclude,high-dose vitamin C can increase the capillary density and retention quality of autologous fat,reduce the degree of inflammatory infiltration and fibrosis,and reduce the formation of vesicles in rats.