摘要
Objective To observe the effects of miR-21 knockout on proliferation and drug resistance in K562/G01 cells,and to preliminarily explore the mechanism of imatinib sensitivity by knocking out miR-21 in K562/G01 cells.Methods Using CRISPR/Cas9 to knock out the miR-21 gene in K562/G01 cells,and single-cell-derived clones of miR-21 knockout were obtained by genomic DNA PCR screening.
作者
ZHANG YUN
张雲(Fujian Med Univ Union Hosp,Fujian Hematol Instit,Fujian Prov Key Hematol Lab,Fuzhou 350001)
