白细胞介素8受体可提高脐带间充质干细胞迁移和向损伤内皮的黏附

Interleukin-8 receptor enhances the migration and adhesion of umbilical cord mesenchymal stem cells to injured endothelium

背景:内皮功能障碍是动脉粥样硬化和心血管疾病的早期预测因子,脐带间充质干细胞移植为治疗血管损伤提供了可能性。目的:探讨过表达白细胞介素8受体A/B对人脐带间充质干细胞迁移能力的影响。方法:培养原代人脐带间充质干细胞和人脐静脉内皮细胞,转染含白细胞介素8受体A/B的腺病毒载体,荧光共聚焦和Western blot检测转染后细胞中白细胞介素8受体A/B的表达,然后通过CCK-8实验、竞争实验和黏附实验检测过表达白细胞介素8受体A/B对细胞增殖能力、竞争能力和迁移能力的影响。结果与结论:过表达白细胞介素8受体A/B的荧光腺病毒载体对细胞增殖能力无明显影响,但过表达白细胞介素8受体A/B的人脐带间充质干细胞竞争和迁移能力明显高于过表达白细胞介素8受体A/B的人脐静脉内皮细胞(P<0.05)。结果表明,白细胞介素8受体提高了人脐带间充质干细胞的迁移能力,促进向损伤内皮的黏附。

BACKGROUND:Endothelial dysfunction is an early predictor of atherosclerosis and cardiovascular disease.Umbilical cord mesenchymal stem cell transplantation provides the possibility for the treatment of vascular injury.OBJECTIVE:To explore the effect of overexpression of interleukin-8 receptor A/B on migration of human umbilical cord mesenchymal stem cells.METHODS:Primary cultured human umbilical cord mesenchymal stem cells and human umbilical vein endothelial cells were transfected with adenovirus vector containing interleukin-8 receptor A/B.Confocal immunofluorescence microscopy and western blot assay were used to observe the expression of interleukin-8 receptor A/B in cells.CCK-8 assay,competition test and adhesion test were used to detect the effects of interleukin-8 receptor A/B overexpression on cell proliferation,competitiveness and migration.RESULTS AND CONCLUSION:The fluorescent adenovirus vector overexpressing interleukin-8 receptor A/B has no significant effect on cell proliferation.However,the competition and migration abilities of human umbilical cord mesenchymal stem cells overexpressing interleukin-8 receptor A/B were significantly higher than those of human umbilical vein endothelial cells overexpressing interleukin-8 receptor A/B(P<0.05).It is concluded that interleukin-8 receptor enhances the migration ability of human umbilical cord mesenchymal stem cells and promotes adhesion to injured endothelium.