基于表型性状和SSR标记的9份万寿菊种质遗传多样性分析

Genetic Diversity Analysis of 9 Marigold Germplasms Based on Phenotypic Traits and SSR Markers

基于9个形态学标记和11个SSR分子标记对9个万寿菊雄性不育两用系进行性状间主成分分析,材料间聚类分析,构建指纹图谱,并计算材料性状之间的变异系数和多样性指数。结果表明,9个万寿菊雄性不育两用系各表型性状具有明显差异,株高、冠幅、花朵数、一级分枝数、花径、千粒重和开花前期在品系间差异均达到极显著水平(p<0.01)。9个两用系的Shannon-Wiener信息指数为1.49。主成分分析结果显示,前3个主成分因子主要包括株高、冠幅、一级分枝数、花朵数等性状,累计贡献率达到了83.348%。11对SSR引物共检测到29个条带,其中27个具有多态性,多态性比率为93.1%,每对SSR引物可扩增条带数2~4条,平均每个位点等位基因数为2.45个。Shannon信息指数和PIC值分别为0.6613和0.353,表型及SSR标记显示9个材料具有较高的遗传多样性,且两种聚类结果总体上具有一致性。3个SSR引物将9个两用系完全区分开来,并构建了供试材料的数字指纹图谱。

In this paper,the 9 marigold male sterile lines were used as materials to conduct principal component analysis,cluster analysis,fingerprint construction and the variation coefficient and diversity index of traits among these materials were also analyzed.The results showed that there were significant differences in plant height,canopy,flower number,number of first-order branch,flower diameter,1000-seed weight and early flowering stage among the 9 marigold male sterile lines(p<0.01),and Shannon-Wiener information index of 9 male sterile lines was 1.49.Principal component analysis showed that the first three principal component factors mainly included plant height,canopy,number of first-order branch,flower number and other traits,with a cumulative contribution rate of 83.348%.A total of 29 bands were detected from 11 pairs of SSR primers,of which 27 were polymorphic,with a polymorphism ratio of 93.1%.Each pair of SSR primers could amplify 2 to 4 bands,and the average number of alleles per locus was 2.45.In addition,Shannon information index and PIC value were 0.6613 and 0.353,respectively.Phenotypic and SSR markers showed that the 9 materials had high genetic diversity,and the two clustering results were consistent in general.And,the 9 male sterile lines were completely separated by 3 SSR primers,and the fingerprints of these materials were also constructed.