摘要
以云南省红河州金平县采集的小叶龙竹(Dendrocalamus barbatus)种子为材料,利用人工气候箱进行高温高湿(相对湿度95%,温度40℃)的人工老化处理,以新鲜采收的种子作为对照,分别测定发芽率、发芽势、含水量等生长指标,以及电解质外渗率(REC)、游离脯氨酸(Pro)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性等生理生化指标。结果表明,在人工老化处理19 d期间,小叶龙竹种子含水量、发芽率和发芽势均随老化天数的增加呈不断下降的趋势,其REC和伤害度总体呈上升趋势,而Pro含量、SOD活性和POD活性均呈先上升后下降的变化趋势,并在老化第7天达到峰值,表明小叶龙竹种子在人工老化前期通过调节渗调作用和保护酶活性来维持相对较高的种子活力。
In this study,the seeds of Dendrocalamus barbatus collected from Jinping County,Honghe Autonomous Prefecture,Yunnan Province,were subjected to artificial aging treatment with high temperature and high humidity(95%relative humidity,temperature 40℃)in an artificial climate chamber with freshly harvested seeds as control.And,growth indexes including germination rate,germination potential,water content,electrolyte leakage rate(REC),free proline(Pro)content,superoxide dismutase(SOD)activity,peroxidase(POD)activity were determined.The results showed that during 19 days of artificial aging,the water content,germination rate and germination potential of the D.barbatus seeds decreased with the increase of aging days,and the REC and damage degree of the seeds increased.However,the Pro content,SOD activity and POD activity all increased first and then decreased,and reached the peak value on the 7 th day of aging,indicating that the seed vigor of D.barbatus maintained relatively high by regulating osmotic regulation and protective enzyme activity in the early stage of artificial aging.
作者
罗玛妮娅
张艺
普甜
郭跻敏
王娟
杨宇明
郑元
LUO Maniya;ZHANG Yi;PU Tian;GUO Jimin;WANG Juan;YANG Yuming;ZHENG Yuan(College of Forestry,Southwest Forestry University,Kunming Yunnan 650224,China;Institute of Green Development,Southwest Forestry University,Kunming Yunnan 650233,China)
出处
《种子》
北大核心
2021年第10期85-89,共5页
Seed
基金
云南省农业基础研究联合专项(2017 FG 001(-082))
云南省高校优势特色重点学科(生物学)建设项目“国家林业局云南珍稀濒特植物保护与繁育重点实验室开放基金”(2016-01)。
关键词
小叶龙竹
种子萌发
人工老化
生理生化特性
Dendrocalamus barbatus
seed germination
artificial aging
physiological and biochemical characteristics