电针人迎、太冲穴对自发性高血压大鼠模型肾脏组织FoxP3和RORγt的影响

Effect of electroacupuncture at Renying and Taichong on kidney tissue Foxp3 and RORγt in spontaneous hypertension rats model

[目的]观察“活血散风”针刺法对高血压前期大鼠肾脏组织叉头转录因子(FoxP3)和维甲酸相关孤独核受体(RORγt)蛋白和mRNA表达的影响。[方法]将16只4周龄自发性高血压大鼠(SHRs)随机分为模型组(SHR组)和电针组(电针+SHR组),每组8只,另将8只同周龄WKY大鼠作为空白对照组(WKY组)。电针组给予针刺人迎、太冲治疗,每日1次,连续治疗2周。采用Softron无创血压仪测量干预前、干预1周、干预2周时的收缩压水平,苏木精-伊红(HE)染色观察大鼠肾脏结构形态,蛋白免疫印迹(Western blot)和聚合酶链反应(PCR)技术检测大鼠肾脏组织FoxP3、RORγt蛋白和mRNA表达情况。[结果]SHR大鼠收缩压水平明显高于WKY组(P<0.05),干预两周后,电针+SHR组收缩压水平明显低于SHR组(P<0.05);SHR组肾脏组织RORγt mRNA表达明显高于WKY组(P<0.05),FoxP3蛋白和mRNA的表达、FoxP3/RORγt比值明显低于WKY组(P<0.05);电针+SHR组肾脏组织RORγt mRNA表达明显低于SHR组(P<0.05),FoxP3蛋白表达、FoxP3/RORγt比值明显高于SHR组(P<0.05);电针干预后SHR大鼠未出现明显的肾脏形态改变。[结论]“活血散风”针刺法可调节FoxP3和RORγt的表达,其对高血压前期大鼠的降压作用可能与调节FoxP3和RORγt的平衡有关。

[Objective]To observe the effect of“Huoxue Sanfeng”acupuncture on the protein and mRNA expression of FoxP3 and RORγt in kidney tissue of prehypertensive rats.[Method]Sixteen 4-week-old spontaneously hypertensive rats were randomly divided into a model group(SHR group)and an electro-acupuncture group(EA+SHR group),8 rats in each group.8 WKY rats served as a blank control group.In EA+SHR group,acupuncture were performed at Renying and Taichong bilaterally,once a day for 2 weeks.The Softron non-invasive blood pressure measurement was used to measure the systolic blood pressure before intervention,1 week and 2 weeks after EA treatment.HE staining was used to detect the morphology of rats renal.Western blot and PCR technology were applied to assessed the FoxP3,RORγt protein and mRNA expression.[Result]The level of systolic blood pressure in SHR rats was significantly higher than that in WKY group(P<0.05).After two weeks of intervention,the level of systolic blood pressure in EA+SHR group was significantly lower than that of SHR group(P<0.05).The expression of RORγt mRNA in kidney tissue of SHR group was significantly higher than that of WKY group(P<0.05),FoxP3 protein and mRNA expression,as well as the FoxP3/RORγt ratio was significantly lower than that of WKY group(P<0.05).The renal tissue RORγt mRNA expression of EA+SHR group was significantly lower than that of SHR group(P<0.05),FoxP3 protein expression and FoxP3/RORγt ratio were significantly higher than that of SHR group(P<0.05).There was no obvious change of renal morphology after EA intervention.[Conclusion]“Huoxue Sanfeng”acupuncture can regulate the expression of FoxP3 and RORγt,and the antihypertensive effect of EA on prehypertensive rats may be related to regulating the balance of FoxP3 and RORγt.