黄海海域耐麻痹性贝毒微生物的筛选及初步鉴定

Screening and Preliminary Identification of Microorganisms Resistant to Paralytic Shellfish Toxin in the Yellow Sea

麻痹性贝类毒素(Paralytic Shellfish Toxin,PST)是来源于有害藻类的强效神经毒素,可在海洋生物体内积累且易通过食物链传递,对人类健康存在着潜在的危害。以大连黄海海域野生牡蛎为原材料,采用平板涂布法分离出X1,X2,X3,X4等4株可能能够耐麻痹性贝毒-N-磺酰氨甲酰基类毒素(CRM-C1&2-b毒素)的菌株,进一步将其分别在不含毒素和含有不同含量毒素的海生菌肉汤液体培养基中共培养48 h,绘制生长曲线并进行对比分析。结果表明,X1和X3菌株具有较弱的耐CRM-C1&2-b毒素能力;X2菌株可能具有分解利用CRM-C1&2-b毒素的能力;X4具有较强的耐CRM-C1&2-b毒素的能力。通过形态学观察,判断X2和X4均为革兰氏阴性细菌,X2为杆菌,X4为球菌。本研究旨在为海洋麻痹性贝类毒素污染防治及降解措施的研究奠定基础。

Paralytic shellfish toxin(PST)is a potent neurotoxin derived from harmful algae,which can accumulate in marine organisms and be easily transmitted through the food chain.Four strains of X1,X2,X3 and X4 which may be resistant to PST-N-sulfonyl carbamoyl toxin(CRM-C1&2-b toxin)are isolated from wild oysters in the Yellow Sea area of Dalian by using spread plate method,the strains are cultured for 48 hours in the liquid culture medium without or with different toxin contents,and the growth curves are drawn and compared.The results shows that X1 and X3 strains have weak resistance to CRM-C1&2-b toxin,X2 might have the ability to decompose and utilize CRM-C1&2-b toxin,and X4 might have the ability to resist CRM-C1&2-b toxin.By morphological observation,X2 and X4 are identified as Gram-negative bacteria,X2 is bacilli and X4 is coccus.The purpose of this study is to lay a foundation for the study of marine paralytic shellfish toxin pollution control and degradation measures.