全烟气对细胞抗氧化能力的影响

Research on the Effects of Whole Smoke on Antioxidant Capacity of Cells

卷烟全烟气暴露V79细胞,选取暴露时长1 h不同浓度(4支烟/h、6支烟/h、8支烟/h)组,以活性氧(ROS)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、总抗氧化能力(T-AOC)为指标,研究全烟气对细胞抗氧化能力的影响。结果表明,全烟气会对细胞内的抗氧化物质产生消耗和抑制,导致胞内的活性氧自由基含量增加,抑制的效果随烟气浓度的增加而愈加显著。

V79 cells were exposed to whole cigarette smoke for 1 hour with 4 cigarettes per hour,6 cigarettes per hour and 8 cigarettes per hour respectively.ROS,SOD,GSH-Px,CAT and T-AOC were taken as index,and the effects of cigarette smoke on the antioxidant capacity of V79 cells were studied.The results showed that the whole smoke environment will consume and inhibit the antioxidant substance in the cell,which leads to the increase of the content of active oxygen free radical in cells.