一株抗小麦赤霉病菌的白莲蒿内生真菌的筛选及代谢产物质谱分析

Analysis on the Screening of an Endophytic Fungi from Artemisia Sacrorum Ledeb Against Wheat Scab and Mass Spectrographic Analysis

采用表面消毒法和尖端菌丝挑取法从白莲蒿中分离纯化12株内生真菌菌株;采用菌丝生长速率法研究8株内生菌发酵液的抗小麦赤霉病活性;采用形态学结合ITS rDNA区基因序列鉴定活性最好菌株为芳香镰刀菌Fusarium redolens,并建立质谱方法分析其主要代谢产物。结果表明:白莲蒿内生真菌(No:20202707)对小麦赤霉病病原菌禾谷镰刀菌的抑菌作用最强,抑菌率在65.27%。其主要代谢产物主要有3,4-二氢香豆素、5′脱氧核糖核苷、Harmala生物碱、苯并呋喃、苯并噻唑等。

Twelve endophytic fungus strain from artemisia sacrorum ledeb were isolated and purified with the method of surface disinfection and tip mycelium selection.The activity of eight strains of endophytic fermentation broth against wheat scab were studied by mycelial growth rate method.Most active strain were identified as Fusarium Redolens by the combination of morphology and ITS rDNA gene sequence.A mass spectrometry method was established to analyze the main metabolites.The results showed that the endophytic fungus(No:20202707)from artemisia sacrorum ledeb demonstrated the strongest inhibitory effect on Fusarium graminearum,and the inhibitory rate was 65.27%.Its main metabolites were 3,4-dihydrocoumarin,5′deoxyribonuc leoside,Harmala alkaloid,benzofuran and benzothiazole.