温度和铜绿微囊藻浓度对萼花臂尾轮虫生活史参数的影响

Effects of Temperature and Microcystis aeruginosa Concentration on the Life History Parameter of Brachionus calyciflorus

[目的]了解温度和铜绿微囊藻浓度对轮虫生活史的影响,以萼花臂尾轮虫为研究对象进行了生命表研究。[方法]在15、25、35℃和不同斜生栅藻浓度下,探究铜绿微囊藻对萼花臂尾轮虫生活史的影响。[结果]温度、斜生栅藻浓度以及二者交互作用下,铜绿微囊藻均对萼花臂尾轮虫的净生殖率(R_(0))、世代时间(T)和内禀增长率(r_(m))有显著影响(P<0.05)。当斜生栅藻浓度分别为10~5、10~6 cells/mL时,处理组相对于对照组,温度在15℃时,R_(0)分别下降了9.4%、69.3%,r_(m)分别下降了20.0%、63.6%,铜绿微囊藻在斜生栅藻浓度充足时对萼花臂尾轮虫繁殖与生长的抑制作用要大于斜生栅藻浓度紧缺时;在温度25℃时,R_(0)分别下降了66.3%、45.6%,r_(m)分别下降了77.8%、22.2%,铜绿微囊藻对萼花臂尾轮虫的抑制作用随着斜生栅藻浓度的提升而减小;温度在35℃时,R_(0)分别下降了71.8%、83.0%,r_(m)分别下降了90.0%、70.7%,铜绿微囊藻对轮虫的抑制作用随着斜生栅藻浓度的提升而加大。[结论]铜绿微囊藻对轮虫的作用受其他可食性绿藻食物浓度的影响,且这种影响会随着温度的变化而变化。该研究通过测定不同温度和不同食物浓度间的交互作用对萼花臂尾轮虫一些重要的生活史参数和生命表因子的影响来了解其基本生物学特征以及为以后研究野外环境中各种因子对轮虫影响中所起的作用做好数据与理论的铺垫。

[Objective]To understand the influence of temperature and Microcystis aeruginosa concentration on the life cycle of rotifers,the life table experiment was carried out with the Brachionus calyciflorus as the research object.[Method]The effects of Microcystis aeruginosa on the life cycle of Brachionus calyciflorus were investigated at 15、25、35℃and different concentrations of Scenedesmus obliquus.[Result]Temperature,the concentration and the interaction of the two had significant effects on the net reproductive rate(R_(0)),generation time(T)and intrinsic growth rate(r_(m))of the Brachionus calyciflorus(P<0.05).When the concentrations of Scenedesmus obliquus were 105 and 106 cells/mL,R_(0) decreased by 9.4%and 69.3%,and r_(m) decreased by 20.0%and 63.6%,respectively,in the treatment group compared with the control group,when the temperature was 15℃.The inhibition effect of Microcystis aeruginosa on the reproduction and growth of Brachionus calyciflorus was greater when the concentration of Scenedesmus obliquus was sufficient than when the concentration of Scenedesmus obliquus was deficient.When the temperature was 25℃,R_(0) decreased by 66.3%and 45.6%,respectively,and r_(m) decreased by 77.8%and 22.2%,respectively.The inhibitory effect of Microcystis aeruginosa on Brachionus calyciflorus decreased with the increase of the concentration of Scenedesmus obliquus.When the temperature was 35℃,R_(0) decreased by 71.8%and 83.0%,and r_(m) decreased by 90.0%and 70.7%,respectively.The inhibitory effect of Microcystis aeruginosa on rotifers increased with the increase of the concentration of Scenedesmus obliquus.[Conclusion]The results showed that the effects of Microcystis aeruginosa on rotifers were influenced by the concentrations of other edible green algae,and the effects varied with the change of temperature.In this study,we hope to find out some basic biological characteristics of Brachionus calyciflorus by measuring the effect of the interaction between different temperature and food concentration on some important life history parameters and life table factors to understand the future research field environment various factors influence on rotifers,the role of well data and theory foreshadowing.