组织修复和再生领域浓缩生长因子的应用优势

Application advantages of concentrated growth factors in the field of tissue repair and regeneration

背景:浓缩生长因子作为最新一代自体血小板浓缩生物制剂,因其具有天然的纤维蛋白支架和丰富的内源性生长因子,近年来已成为组织修复和再生领域的研究热点。目的:总结浓缩生长因子在组织修复和再生领域中的研究进展,探讨目前研究阶段存在的问题,以期为浓缩生长因子的后续研究提供新的参考思路。方法:文章以“浓缩生长因子、干细胞、组织修复、组织再生”为中文检索词,以“Concentrated growth factor,CGF,Stem cells,Tissue repair,Tissue regeneration”为英文检索词,在PubMed、EMbase、Medline和中国知网数据库进行全面检索。收集归纳近10年来浓缩生长因子在组织修复和再生领域应用的相关研究证据,根据严格纳入排除标准筛选文献,最终纳入96篇。结果与结论:①文章从浓缩生长因子制备和应用的具体方法,促进组织修复和再生的原理,体外对细胞增殖和分化的作用,以及体内对组织修复和重建4个方面详细总结了浓缩生长因子目前的研究进展,讨论了现阶段存在的问题和未来可能的研究发展方向。②浓缩生长因子是一个在组织修复和再生领域具有潜力的自体生物活性因子,制备方法简便,应用方式灵活多变,完全取自自体血液,无免疫排斥反应,生物相容性良好。③浓缩生长因子的核心结构包括天然的纤维蛋白支架和多种内源性生长因子,在体外能促进多种干细胞的增殖、迁移、分化,浓缩生长因子与干细胞的复合体是干细胞治疗领域一种新的策略。④浓缩生长因子在体内能促进骨缺损的修复,其与组织工程材料联合所构建的局部生长因子缓释系统是延长生长因子缓释时间的有效方法。⑤浓缩生长因子在促进软组织修复、抗皮肤光老化、减少术后并发症等领域呈现出良好的效果,其已成为目前自体生物活性制剂个性化治疗的优先选择之一。

BACKGROUND:As the latest generation of autologous platelet concentrate biologics,concentrated growth factor has become a research hotspot in the field of tissue repair and regeneration due to its natural fibrin scaffold and abundant endogenous growth factors.OBJECTIVE:To summarize the research progress of concentrated growth factors in the field of tissue repair and regeneration,as well as discuss the existing problems in the current research stage,in order to provide new reference ideas for the follow-up research of concentrated growth factors.METHODS:This article used“concentrated growth factor,CGF,stem cells,tissue repair,tissue regeneration”as search terms.A comprehensive search was conducted on PubMed,EMbase,Medline,and CNKI.The relevant research evidence of the application of concentrated growth factor in the field of tissue repair and regeneration in the past 10 years were collected and summarized.The literature was screened based on strict inclusion and exclusion criteria.Finally,96 articles were included.RESULTS AND CONCLUSION:(1)This article summarized the research progress of concentrated growth factor from four aspects:the specific method of preparation and application of concentrated growth factor,the principle of concentrated growth factor promoting tissue repair and regeneration,the effect on cell proliferation and differentiation in vitro,and the repair and reconstruction of tissue in vivo,and discussed the current research the existing problems.(2)Concentrated growth factor is an autologous bioactive factor with potential in the field of tissue repair and regeneration.The preparation method is simple and the application method is flexible and changeable.It is completely taken from autologous blood,so that it has great biocompatibility and few immune rejection reactions.(3)The core structure of concentrated growth factor includes a natural fibrin scaffold and a variety of endogenous growth factors,which can promote the proliferation,migration and differentiation of a variety of stem cells in vitro.(4)Concentrated growth factors can promote the repair of bone defects in vivo,and the local growth factor slow-release system constructed by combining it with tissue engineering materials is an effective way to extend the slow-release time of growth factors.(5)Concentrated growth factors have shown great effects in promoting soft tissue repairing,anti-skin photoaging,and reducing postoperative complications.It has become one of the preferred options for personalized treatment of autologous bioactive preparations.