2015-2019年湖北省流行性腮腺炎病例腮腺炎病毒基因特征

Genetic characteristics of mumps virus strains circulating in Hubei province,2015-2019

目的分析2015-2019年湖北省流行性腮腺炎(流腮)病例的腮腺炎病毒(Mumps virus,MuV)基因特征。方法收集2015-2019年湖北省流腮病例咽拭子标本,采用荧光定量RT-PCR方法检测MuV核酸、扩增SH基因并进行序列分析;对核酸阳性标本进行MuV分离培养,对分离株进行全基因序列分析。结果共获得22例流腮病例的MuV SH基因序列,其中16例为F基因型(2015年11例、2017年4例、2019年1例),6例为G基因型(均为2019年病例);分离到6株MuV毒株,其中5株为F基因型,1株为G基因型。16株F基因型MuV和6株G基因型MuV的SH基因核苷酸同源性分别为94.9%-100%和99.4%-100%。6株MuV分离株与中国疫苗株S79和Jeryl-lynn株的核苷酸同源性为92.9%-93.1%。结论2015-2019年湖北省MuV优势基因型为F基因型,2019年首次发现G基因型且为当年流行优势株。需继续开展病原学监测,为流腮防控提供实验室依据。

Objective To determine the genetic characteristics of mumps viruses(MuV)circulating during 2015-2019 in Hubei province.Methods We collected throat swab specimens from mumps cases during 2015-2019 in Hubei and used fluorescence quantitative RT-PCR to detect MuV nucleic acid and amplify SH genes for sequence analyses.We isolated mumps viruses from nucleic acid positive specimens for whole genome sequencing.Results SH gene sequences were obtained from 22 mumps cases,among which 16 were F genotype(11 in 2015,4 in 2017,1 in 2019)and 6 were G genotype(all in 2019).Six mumps viruses were isolated;5 were F genotype and 1 was G genotype.The SH gene nucleotide homologies within the 16 F genotype strains and within the 6 G genotype strains were 94.9%-100%and 99.4%-100%,respectively.Compared with the Chinese vaccine strain S79 and the Jeryl-Lynn strain,the nucleotide homologies of the 6 MuV isolates were 92.9%-93.1%.Conclusions The F genotype MuV strain was predominant during 2005-2019 in Hubei;the G genotype was first discovered and became predominant in 2019.We should enhance etiological surveillance to provide laboratory support for mumps prevention and control.