纳米磁热复温玻璃化低温保存大鼠肾脏的研究

Study on Magnetic Induction Nanowarming of Vitrified Whole Rat Kidneys

纳米磁热复温有望解决器官玻璃化低温保存复温的难题。本文测试了氧化铁纳米颗粒在VS55低温保护剂溶液中的胶体稳定性及磁热性能。结果表明,纳米颗粒VS55溶液分散体系即使在玻璃化冷冻磁热复温后,仍在较长时间内保持胶体稳定,且磁热性能随交变磁场电流、纳米颗粒浓度的增加而提升。磁共振成像结果显示通过灌注能够基本实现纳米颗粒在大鼠肾脏组织中的均匀分布与完全洗脱。肾脏总体结构在玻璃化冷冻磁热复温后保持完整,但磁共振成像显示其内部血管网络可能受到一定程度的损伤。研究结果说明,通过进一步提升磁场强度或纳米颗粒的磁热性能,优化灌注加载流程,有望实现更加快速均匀的复温,从而在不久的将来解决器官玻璃化保存的复温难题。

Magnetic nano-rewarming is a promising approach to solve the problem of long-term vitrification preservation of organs at deep low temperature.Here,we test the colloid stability and the heating properties of magnetic iron oxide nanoparticles in VS55 cryoprotectant solutions.The results show that magnetic VS55 solutions remain colloidally stable for a long time even after vitrification and nano-rewarming.The heating properties of magnetic VS55 increased with the increasing of alternating magnetic field current and nanoparticle concentrations.MRI results show that the uniform loading and complete wash out of nanoparticles in rat kidney tissues can be basically achieved by perfusion.After vitrification and nano-rewarming,the overall structure of the whole rat kidney remains intact while the internal blood vessel network may be partially damaged.This work demonstrates that the faster and more uniform rewarming can be achieved by optimizing the perfusion loading process,improving the magnetic field strength or the nanoparticle heating performance,etc.Thus,the challenge of organ vitrification cryopreservation rewarming may be solved in the near future.