星形电极介电泳芯片

Star-shape Electrode Dielectrophoresis Chip

提出了一种利用负介电泳力在介电泳芯片上富集聚苯乙烯(PS)微球的有效方法:采用lift-off工艺在玻璃衬底上设计并制作了星形结构的微电极,在高频交流信号作用下,星形电极芯片能够提供一个特定的非均匀电场,从而产生负介电泳力。研究了交流信号的幅值和频率、工作液电导率和浓度等参数对PS微球富集效果的影响。结果显示,在交流信号的电压参数VP-P为9 V,频率参数为1 MHz时,低电导率PS微球悬浮液添加到介电泳芯片上时,PS微球在电极中心区域高效率富集。在负介电泳力辅助下,浓度范围在0.33 amol/mL~2.08 amol/mL的PS微球悬浮液内可形成微球团簇状态。研究结果在分子细胞生物学、药理学和生物化学有潜在的应用前景。

In this paper,an effective method of enriching polystyrene(PS)microspheres on dielectrophoretic chip by negative dielectrophoretic force was proposed.A star-shaped microelectrode was designed and fabricated on a glass substrate by a lift-off process.Under the action of a high-frequency AC signal,the star-shaped microelectrode chip can provide a specific non-uniform electric field,which can produce negative dielectrophoretic force.The effects of amplitude and frequency of AC signal,conductivity and concentration of working solution on the enrichment of PS microspheres were investigated.The results show that when the voltage parameter of AC signal is 9 V and the frequency parameter is 1 MHz,the PS microspheres with low conductivity are enriched in the center of the electrode.With the aid of negative dielectrophoresis force,the concentration of PS microsphere suspension in the range of 0.33~2.08 amol/mL can form microsphere clusters.The results of this study have potential applications in molecular cell biology,pharmacology,and biochemistry.