Juvenile hormone receptor Met regulates sleep and neuronal morphology via glial-neuronal crosstalk

Juvenile hormone(JH)is one of the most important hormones in insects since it is essential for insect development.The mechanism by which JH affects the central nervous system still remains a mystery.In this study,we demonstrate that one of the JH receptors,Methoprene-tolerant(Met),is important for the control of neurite development and sleep behavior in Drosophila.With the identification of Met-expressing glial cells,the mechanism that Met negatively controls the mushroom body(MB)b lobes fusion and positively maintains pigment-dispersing factor s LNvs projection pruning has been established.Furthermore,despite the developmental effects,Met can also maintain nighttime sleep in a developmentindependent manner through the a/b lobe of MB.Combining analyses of neuronal morphology and entomological behavior,this study advances our understanding of how the JH receptor regulates the nervous system.