基于酶标仪测定的斯氏油脂酵母发酵产油脂条件优化研究

Research on the Conditions Optimization of Lipid Production Through Lipomyces Starkeyi Fermentation by Spectrophotometry with Enzyme-Labeled Instrument

以斯氏油脂酵母为研究对象,利用苏丹黑B染色和全自动酶标仪分光光度法测定细胞内油脂含量。通过监测发酵过程中苏丹黑B染色后的OD最大吸收峰值、菌体密度、发酵液还原糖含量的变化,探讨氮源、碳氮比和接种量对斯氏油脂酵母发酵产油脂量的影响。结果表明,发酵产油脂的最佳氮源为硫酸铵,最佳碳氮比为66∶1,最佳接种量为10%。

With Lipomyces starkeyi as the research object,the lipid content is determined by staining with the Sudan black B and spectrophotometry with enzyme-labeled instrument.By monitoring the changes of the peak absorption value of OD,density of the bacterial strain,reducing sugar content in the fermentation broth after the Sudan black B staining during the fermentation process,the effects of nitrogen source,carbon-nitrogen ratio and inoculation quantity on the lipid production of Lipomyces starkeyi is studied.The results show that the best nitrogen source of fermentation is ammonia sulfate,the best carbon to nitrogen ratio is 66∶1,and the best inoculation quantity is 10%.