乙醇对HepG2细胞VASN蛋白表达与分泌水平的影响研究

Effect of ethanol on the expression and secretion of vasn in HepG2 cells

目的观察乙醇刺激下HepG2细胞VASORIN蛋白(VASN)的变化,分析乙醇对HepG2细胞VASN蛋白表达与分泌水平的影响。方法使用不同浓度乙醇处理液对HepG2细胞进行刺激,分别在24、48、72 h收集细胞,应用荧光定量核酸扩增检测系统(qPCR)检测HepG2细胞VASN信使核糖核酸(mRNA)水平,取超滤浓缩后的样品进行分泌水平检测,分析乙醇对HepG2细胞VASN蛋白表达与分泌水平的影响。结果与无乙醇刺激比较,25 mmol/L浓度乙醇刺激、50 mmol/L浓度乙醇刺激、100 mmol/L浓度乙醇刺激在24、48、72 h下HepG2细胞VASNmRNA水平均明显较高;与无乙醇刺激比较,25 mmol/L浓度乙醇刺激、50 mmol/L浓度乙醇刺激、100 mmol/L浓度乙醇刺激在24、48、72 h下HepG2细胞VASN蛋白分泌水平均明显较高。结论乙醇能促进HepG2细胞VASN蛋白表达与分泌水平的提高,可为生物学细胞研究等提供有效参考。

Objective To observe the change of vasn protein(VASN)in HepG2 cells stimulated by ethanol and analyze the effect of ethanol on the expression and secretion of VASN protein in HepG2 cells.Methods HepG2 cells were stimulated with different concentrations of ethanol,and collected at 24 h,48 h and 72 h,respectively.The VASN mRNA level of HepG2 cells was detected by quantitative nucleic acid amplification detection system(qPCR).The secretory level of samples after ultrafiltration concentration was detected,and the effect of ethanol on the VASN protein expression and secretion level of HepG2 cells were analyzed.Results Compared with no ethanol stimulation,the VASN mRNA level of HepG2 cells stimulated by 25 mmol/L ethanol,50 mmol/L ethanol and 100 mmol/L ethanol was significantly higher at 24 h,48 h and 72 h;compared with no ethanol stimulation,the VASN protein secretion level of HepG2 cells stimulated by 25 mmol/L ethanol,50 mmol/L ethanol and 100 mmol/L ethanol was significantly higher at 24 h,48 h and 72 h.Conclusion Ethanol can promote the expression and secretion of VASN in HepG2 cells,and provide an effective reference for biological cell research.